摘要
我们以糖皮质激素受体(GR)的竞争性拮抗剂RU38486(简称RU486)阻断大鼠体内的GR.并通过检测肺、肾组织匀浆中荧光标记白蛋白的含量,观察了烫伤后12h大鼠肺、肾血管壁通透性的变化以及阻断GR对这种变化的影响。结果显示:烫伤后12 h,大鼠肺、肾组织匀浆中荧光标记白蛋白含量明显高于对照组(肺:P<0.05;肾:P<0.001);阻断GR后再烫伤大鼠,其肺、肾组织匀浆中的荧光白蛋白含量又显著高于烫伤组(P<0.05)。提示:①烫伤后12 h,大鼠肺、肾血管壁通透性明显升高。②GR减少可加重烫伤所致的血管壁通透性升高.并可逆转内源性糖皮质激素(GC)稳定血管壁通透性的作用。
With glucocorticoid receptor(GR)blockade by RU38486,a competitive antagonist of GR,the change in contents of FITC labeled albumin(FITC—albumin)in plumonary and renal tissue in scalded rats have been measured to study the changes in vascular permeability.The result showed that the contents of FITC-albumin in pul- monary and renal tissue in the scalded rats were markedly higher than those of the con- trols(lung:P<0.05,kidney:P<0.001).When the scalded rats were given GR block- ade,the contents of FITC-albumin in pulmonary and renal tissue were significantly higher than those of rats with scald only(P<0.05).The results indicated:the pul- monary and renal vascular permeability in scalded rats was markedly enhanced;GR blockade might aggravate the increase in vascular permeability caused by scald,so that the protective effect of glucocorticoid(GC)on vascular permeability seemed to be re- versed.
