摘要
为探讨血小板活化因子（ＰＡＦ）在烧伤后肠粘膜屏障损害中的作用。作者在大鼠３０％Ⅲ度烫伤模型上，观察了肠组织ＰＡＦ含量变化与肠粘膜电位（ＰＤ）、肠组织磷脂酶Ａ＿２（ＰＬＡ＿２）活化、髓过氧化酶（ＭＰＯ）活力以及丙二醛（ＭＤＡ）含量变化的关系。结果显示，烫伤后肠组织ＰＡＦ含量显著升高与肠粘膜ＰＤ降低呈显著负相关（ｒ＝一０．９４，Ｐ＜０．０１）。ＰＡＦ拮抗剂ＷＥＢ２１７０可显著抑制烫伤后肠组织ＰＡＦ含量升高，提高肠粘膜ＰＤ，并有效降低肠组织ＰＬＡ＿２、ＭＰＯ和ＭＤＡ水平，从而明显减轻肠粘膜损害。作者认为ＰＡＦ是导致烧伤后肠粘膜屏障损害的重要因素之一，而ＰＬＡ＿２活化、中性白细胞激活以及大量氧自由基释放则是ＰＡＦ导致肠粘膜屏障损害的重要中间机制。
AbstractIn order to investigate the roles of platelet activat-ing factor (PAF)in postburn intestinal barrier injury,the changes in intestinal mucosal potentinal difference(PD),intestinal PAF,Phospholipase A_2 ( PLA_2),myeloperoxidase ( MPO)and malondialdehyde ( MDA )were measured in a model of rats with 30%TBSA Ⅲ° burns. The results showed that the intestinal PAF lev-el was significantly elevated after severe burn and wasnegatively correlated with the decrease of intestinalmucosaI PD(r=一0. 94, P<0.01). PAF antagonistWEB 2170 treatment could significantly inhibite the in-crease of PAF in intestinal tissue, enhance intestinalmucosal PD,attenuate the levels of PLA_2, MPO andMDA in intestinal tissue,and alleviate the pathologicalchanges in ileal mucosa, compared with the burngroup. The results suggest that PAF is an importantfactor causing postburn intestinal mucosal barrier in-jury, and activation of leukocyte and PLA_2 as well asthe release of superoxide are important intermediatemechanisms for PAF leading to intestinal mucosal in-jury.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1995年第7期393-395,共3页
Chinese Journal of Surgery
基金
国家自然科学基金
