摘要
以Ｗｉｓｔａｒ大鼠食管中、下１／３段的冰冻切片为底物，用间接免疫荧光法对１０７例类风湿关节炎、１２０例其他风湿病以及６０例献血者血清检测抗角蛋白抗体（ａｎｔｉｋｅｒａｔｉｎａｎｔｉｂｏｄｉｅｓ，ＡＫＡ）。当角质层显示有明显的呈线形或呈板层状荧光染色图形时，提示为类风湿关节炎的荧光图形，其滴度均在１：８０或以上，特异性为９９％，敏感性为２３％。此抗体的出现与类风湿因子及抗类风湿关节炎３３０００和３６０００抗体阳性无明显相关性，有可能成为类风湿关节炎的又一特异性抗体。
era from 107 patients with rheumatoid arthritis(RA), 1 20 patients with other rheumatie diseasesand 60 blood donors were tested with indrect immunofluoresence on the middle third of Wistar rat oesopha-gus as a substrate for the presence of antikeratin antibodies(AKA). By labelling the stratum corneum andstratum spinosum with IgG antibodies,three patterns of reaction were distinguished. Among them,onlyone pattern which showed an intense linear laminated fluorensence on stratum corneum and a weak fluo-rensence on stratum spinosum was valuable for the diagnosis of RA,with a specificity of 99%and a sensi-tivity of 23%。It was also found that they were not related to the presence of rheumatoid factor or anti-RA3 3 000/3 6 000 antibodies。Thus,AKA might be another marker antibOdy in RA.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1995年第5期330-332,共3页
Chinese Journal of Internal Medicine
