摘要
本文应用免疫印迹技术（ＩＢＴ），以兔胸腺的盐水提取物（ＲＴＥ）为抗原，在类风湿关节炎（ＲＡ）患者血清中检测到与ＲＴＥ中５４ｋＤ蛋白多肽反应的一种新自身抗体，暂命名为抗ＲＡ５４。通过对３６５例各种不同风湿性疾病患者的检测，观察到抗ＲＡ５４主要见于ＲＡ组，阳性率为１４．５％，对ＲＡ的诊断有高度特异性（Ｐ＜０．００１）。
Using immunoblotting technique(IBT) and saline extract of rabbit thymus powder (RTE) as antigen,we identified a new autoantibody to an antigen with a molecular weight of 54000 in the sera of some rheumatoid arthritis (RA) patients. The autoantibody,temporarily called anti-RA54,was mainly found in RA on detecting its presence in 365 patients with various kinds of rheumatic disease. The positivity for anti-RA54 in RA group (n=69) was 14.5%,while that in other rheumatic disease group (n=296) was only 0.7%.So the autoantibody appears to be highly specific for rheumatoid arthritis (P<0.001).
出处
《标记免疫分析与临床》
CAS
1995年第4期197-199,共3页
Labeled Immunoassays and Clinical Medicine
关键词
自身抗体
免疫印迹法
类风湿性关节炎
Arthritis. rheumatoid/diagnosis
Autologous antibody
Immunoblotting
