摘要
目的探讨非小细胞肺癌(NSCLC)组织中上皮钙黏蛋白(Ecadherin)的表达及与预后的关系。方法用组织阵列仪构建365例NSCLC患者手术切除癌组织标本的组织芯片,对该芯片进行Ecadherin免疫组化染色,并分析Ecadherin表达与临床病理资料及生存预后的关系。365例中,鳞癌116例、腺癌199例、腺鳞癌等组织类型50例。结果Ecadherin蛋白主要在肿瘤细胞的胞膜和细胞质中表达,有32.1%(117/365)为Ecadherin低表达。Ecadherin低表达与淋巴结转移(χ2=16.430,P=0.001)、肿瘤细胞低分化(χ2=9.243,P=0.010)以及临床病理分期(χ2=9.421,P=0.024)呈正相关,而与病理类型无明显关系;Ecadherin表达与NSCLC预后差密切相关(P<0.0001)。多因素生存分析表明,Ecadherin表达是NSCLC预后差的独立预后因素(P<0.001)。结论Ecadherin可能与NSCLC的进展有关,其表达状态是NSCLC独立预后因素。
Objective This study was to clarify E-cadherin expressions in non-small cell lung cancer (NSCLC) and its correlation with patients′ prognosis. Methods Tissue microarrays (TMAs) containing specimens from 365 different NSCLC were constructed, covering all stages and almost all histological types of this disease. Slides were immunohistochemically stained with antibodies against E-cadherin. Expression pattern of the protein was analyzed with relation to the clinicopathological. Correlations of the results with patients′ overall survival were also examined. Results Immunohistochemical staining revealed that E-cadherin protein was localized mainly on membranes and the cytoplasm of NSCLC tumors cells. Reduced E-cadherin expression was evident in 32.1%. Reduced E-cadherin expression significantly correlated with lymph nodes metastasis (χ2 = 16.430,P= 0.001), histological dedifferentiation (χ2 = 9.243,P= 0.010) and advanced clinical stage (χ2 = 9.421,P= 0.024). There was no significant difference in E-cadherin expression between squamous cell carcinoma and adenocarcinoma. E-cadherin reduced expression correlated with a poor prognosis (P<0.0001) in univariated analysis. Multivariate analysis showed a significantly lower survival probability for patients with reduced E-cadherin (P< 0.001), and E-cadherin was an independent prognostic factor for survival of NSCLC patients. Conclusions It suggests that dysfunction of E-cadherin has an important impact in the progression of lung cancer. As an independent prognostic factor, expression of E-cadherin can predict outcome of different group, together with conventional prognostic factors, and subsequently make appropriate management.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2005年第14期913-917,共5页
Chinese Journal of Surgery
基金
广州市科学技术局重大科技攻关计划资助项目(2001Z04401)
广东省重点医学科技攻关专项课题资助项目
国家高新技术研究发展计划课题资助项目
