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微小RNA-200c上调E-钙黏蛋白表达对外阴鳞癌A-431细胞侵袭及迁移能力的影响 被引量：1

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摘要 微小RNA（microRNA,miRNA）是一类由长约22个（18～25个不等）核苷酸组成的不编码蛋白质的RNA,通过抑制靶mRNA的翻译或降解靶mRNA来调节基因的功能,从而参与一系列的重要进程.研究表明,miRNA表达异常与多种肿瘤相关,其可以起到肿瘤抑制基因或者癌基因的功能,其功能是在转录后（翻译水平）负调控基因的表达.miRNA对mRNA异常的转录后调节可以促进细胞增殖、抑制细胞凋亡及增强细胞的侵袭能力,从而导致肿瘤的发生和发展.其中,miRNA-200c（miR-200c）与上皮源性肿瘤的发生、发展关系密切.本研究利用基因转染技术将miR-200c转染至外阴鳞癌A-431细胞,通过检测A-431细胞转染前后E-钙黏蛋白（E- cadherin,E-cad）的表达情况及细胞侵袭能力的变化,分析miR-200c对外阴鳞癌A-431细胞侵袭、迁移能力的影响,并初步揭示其可能的机制.
作者 周洋 欧阳玲 李博 宋子璇
机构地区 中国医科大学附属盛京医院妇产科
出处 《中华妇产科杂志》 CAS CSCD 北大核心 2012年第11期864-865,共2页 Chinese Journal of Obstetrics and Gynecology
基金 辽宁省自然科学基金（200920959）
关键词 细胞侵袭能力 E-钙黏蛋白 外阴鳞癌 迁移能力 蛋白表达 肿瘤抑制基因 CADHERIN 基因转染技术
分类号 R737.3 [医药卫生—肿瘤]
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参考文献14

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二级参考文献41

共引文献26

同被引文献23

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中华妇产科杂志
2012年 第11期

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