摘要
目的 采用组织阵列 (tissuearray)技术检测p5 3蛋白在卵巢肿瘤中的表达情况。方法 采用组织阵列技术结合链霉菌抗生物素蛋白 过氧化物酶连接 (SP)法对 5 2例不同卵巢组织进行p5 3蛋白检测。结果 卵巢癌中p5 3蛋白的表达率为 33% ,正常卵巢、卵巢良性肿瘤及卵巢交界性上皮肿瘤中表达率为 0 % (P <0 0 0 5 ) ;p5 3蛋白的过度表达与患者的不同年龄、病理分化程度无显著相关性 (P >0 0 5 ) ,而与不同组织类型具有显著相关性 (P <0 0 5 )。结论 组织阵列技术是研究肿瘤基因的一种新方法 ,具有高效、简便及标准化的特点。p5
Objective To investigate the expression of p53 in human ovarian neoplasms by tissue array technique Methods The expression of p53 protein in various ovarian tissues was studied by tissue array and immunohistochemistry Results The expression rate of p53 was 33% in ovarian cancers There were no expressions in normal ovarian tissues, benign ovarian neoplasms and borderline ovarian neoplasms( P <0 005). p53 expression was not associated with age of patients and grade of differentiation ( P >0 05) while p53 expression was associated with tissue types( P <0 05) Conclusion The expression rates of p53 in ovarian cancers were obviously increased
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2002年第7期405-407,共3页
Chinese Journal of Obstetrics and Gynecology
