摘要
目的分析载脂蛋白E(ApoE)基因多态性及其对慢性乙型肝炎病毒(HBV)感染性免疫的影响。方法白细胞介素(IL)-2检测采用放射免疫法,聚合酶链反应(PCR)法扩增ApoE基因片段,用荧光偏振的方法检测ApoE基因型。结果轻度组ApoEε4高于正常值范围,并与中度、重度组相比差异有统计学意义(P均<0.05),与ApoEε3、ApoEε2组相比ApoEε4组有较高的IL-2和总胆固醇水平。结论高水平的ApoEε4基因可通过调节IL-2和血脂水平减轻HBV感染后引起的肝细胞炎症反应及肝损伤。
Objective The effects of apolipoprotein E (ApoE) gene polymorphism in HBV infective immunity were analyzed. Methods The radioimmunoassay (RIA) was used to detect the quantity of IL-2,PCR,the TDI-FP techniques were separately used to copy the ApoE gene and detect the ApoE gene types. Results The frequency of the ApoEε4 in CHB (chronic hepatitis B) light group was over the normal range, and compared with the mid and severe groups, it was significantly higher (P<0.05). Compared with the ApoEε3 and ApoEε2,the IL-2 and TC in ApoEε4 were higher. Conclusion The high level of ApoEε4 allele may lighten the hepatic inflammatory injury through regulating the IL-2 quantity and plasma lipid level.
出处
《中国药物与临床》
CAS
2005年第7期506-508,共3页
Chinese Remedies & Clinics
