摘要
目的探讨血脂康对THP-1巨噬细胞源性泡沫细胞形成及ATP结合盒转运体A1(ABCA1)和G1(ABCG1)介导的胆固醇外流的影响。方法将THP-1诱导分化成巨噬细胞,再将细胞随机分为空白对照组(正常生长的THP-1细胞),ox-LDL组(100mg/L ox-LDL),血脂康组(100mg/L ox-LDL+100μg/ml血脂康),溶媒组(100mg/L ox-LDL+100μg/ml DMSO),培养48h后测定各组细胞内脂质含量,检测ABAC1和ABCG1 mRNA和蛋白表达的变化,并测定胆固醇外流率。结果用ox-LDL与THP-1巨噬细胞共孵育后,细胞质内有大量脂滴存在,符合泡沫细胞的形态特点。血脂康组泡沫化程度明显减轻。与空白对照组和溶媒组比较,血脂康组细胞内脂质含量明显减少(P<0.01),ABCA1和ABCG1表达水平增加(P<0.05),胆固醇流出率增加(P<0.05)。结论血脂康可通过上调ABCA1和ABCG1的表达来促进胆固醇外流,从而有效抑制泡沫细胞的形成。
Objective To investigate the effects of Xuezhikang capsule on the THP-1 macrophage-derived foam cell formation and the cholesterol efflux mediated by ATP binding cassette transporter A1 （ABCA1） and ATP binding cassette transporter G1 （ABCG1）. Methods THP-1 macrophages were induced to differentiate into macrophage-derived foam cells, and then they were divided into four groups： blank control group, ox-LDL group Xuezhikang-treatment group, and vehicle group. The cells in four groups were co-incubated in culture mediumj with addition of ox-LDL （100μg/ml）, Xuezhikang （100mg/L ox- LDL＋100μg/ml Xuezhikang） and vehicle solution （100mg/L ox-LDL＋100μg/ml DMSO） respectively for 48 hours. The foam cells formation was observed with oil O staining. The contents of intracellular cholesterol and cholesterol ester in foam cells were determined. The mRNA and protein expression levels of ABCA1 and ABCG 1 were determined by qRT-PCR and Western blotting, respectively. In addition, the cholesterol efflux was determined by liquid scintillation. Results After co-incubation with ox-LDL, abundant lipid droplets were found in THP-1 macrophages, which was consistent with the morphological feature of foam cells. Compared with 3 other groups, the foam cell formation was attenuated by the administration of Xuezhikang. Compared with blank control group and vehicle group, the cellular lipid contents in Xuezhikang group significantly decreased （P〈0.01）, while the ABCA1 and ABCG1 expression significantly elevated （P〈0.05）, and the cholesterol efflux significantly promoted by Xuezhikang treatment （P〈0.05）. Conclusions Xuezhikang may inhibit the foam cell formation in vitro by means of reducing intracellular cholesterol level. The cholesterol efflux is enhanced by Xuezhikang, possibly due to up-regulated expression of ABCA1 and ABCG1 in both mRNA and protein level.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2014年第2期116-120,共5页
Medical Journal of Chinese People's Liberation Army
