摘要
目的建立测定丙泊酚血药浓度的高效液相色谱法。方法采用LichrosorbC18色谱柱(4.6mm×200mm,5μm),以甲醇-水(3∶1)为流动相;流速1.0ml/min;荧光检测激发波长276nm;发射波长310nm。以麝香草酚为内标,血清样品用甲醇沉淀蛋白后取上清液进样分析。结果丙泊酚血药浓度在0.2～16.0mg·L-1范围内线性关系良好(r=0.9999),最低检测浓度0.1mg·L-1。低、中、高3个浓度的日内RSD为3.35%～4.19%,日间RSD为3.11%～9.38%。结论本方法简便、准确、精密度好,适用于临床药代动力学研究。
Aim To establish an HPLC method for the determination of propofol concentration in serum.Method The HPLC separation was performed on Lichrosorb C(18) column (4.6mm×200mm,5μm).The mobile phase was methanol-water (3∶1) at a rate of 1.0ml/min.Fluorescence detector: excitation wavelength 276nm,emission wavelength 310nm.The sample treatment for propofol involved a simple protein precipitation with methanol,and thymol worked as internal standard.The supernatant was directly injected into the HPLC system.Results The linear rang was 0.2～16.0mg·L^(-1)(r=0.999 9).The average recovery of propofol was 100.7%±8.12%.RSD of intra-day and inter-day validation were less than 5% and 10%,respectively.The limit detection was 0.1mg·L^(-1).Conclusion The method appeared to be simple,accurale and precise.It is suitable for clinical pharmacokinetics study.
出处
《解放军药学学报》
CAS
2005年第3期229-231,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
