摘要
本文对萝卜软腐欧文氏菌（ＥｒｗｉｎｉａＣａｒｏｔｏｖｏｒａ）生产碱性果胶酶条件进行了研究。结果表明：该菌株的最佳产酶及生长的培养基组成为：果胶０．３０％，谷氨酸钠０．９０％，硫酸铱０．１０％，磷酸二氢钾０．３０％，磷酸氢二钠０．１０％，硫酸镁０．０１０％及氯化钙０．０１０％。其最适发酵（摇瓶）条件为：起始酸度ｐＨ７．２～７．６，温度２８℃，摇瓶转速１８０ｒｐｍ，接种量５％（ｖ／ｖ）及放罐周期１０小时。
he preparation and properties of alkalophilic pectinolytic
enzyme(polygalacturonate trans-elimi-natase, i. e. PATE)from Erwinia Carotovora were
investigated The optimized results showed as thatthe medium concentrations:pectin
0.30%,monosodium L-glutamate 0.90%,(NH4)2SO4 0.10%,KH2PO4 0.0%,Na2HPO4 0.10%,MgSO4
0.01%,CaCL2,0.01%and the initial pH 7.2～7.6,thereaction temperature 28℃,the flask shaking rate
180 rpm,the seed inoculation ratio 5%(v/v)andbatch time 10 hr.
出处
《药物生物技术》
CAS
CSCD
1994年第2期36-39,共4页
Pharmaceutical Biotechnology
基金
中科院广州化学所纤维素化学开放实验室
关键词
欧文氏菌
碱性果胶酶
发酵
制备
性质
E. Carotovora
PATE
Fermentation
Preparation
Property
