摘要
目的通过检测类风湿性关节炎(RA)患者体内氧化还原物水平,探讨氧化还原物水平与类风湿性关节炎发生、发展的关系。方法选择83例RA患者,采用高效液相色谱电化学法检测其血浆总抗坏血酸(TAA)、脱氢抗坏血酸/总抗坏血酸(DHAA/TAA)、维生素E、晚期蛋白质氧化产物(AOPP)、血浆丙二醛(MDA)等氧化还原指标;对RA患者血浆AOPP、MDA及高敏C-反应蛋白(hs-CRP)做相关分析,并以30名健康人作为对照进行统计分析。结果RA患者血浆MDA、AOPP及DHAA/TAA水平显著高于正常人(P<0.05),AOPP增高则极为显著(P<0.01);维生素E与正常人无显著差异(P>0.05)。RA患者MDA与hs-CRP明显相关(P<0.01),AOPP与hs-CRP则无相关性(P>0.05)。结论RA患者体内氧化还原水平明显升高,是RA病程发展的重要机制;AOPP、MDA两项指标可反映RA患者体内的高氧化水平。
Objective To examine the oxidation or reduction products in patients with rheumatism arthritis (RA), and inves- tigate the relationship between oxidation or reduction products and occurrence and development of RA. Methods The serum levels of total ascorbic acid (TAA), dehydroascorbic acid (DHAA)/TAA, vitamin E, advanced oxidation protein products (AOPP) and malondialdehyde (MDA) were detected by high-performance liquid chromatography with electrochemical detection in 83 RA patients and 30 healthy adults. Correlation analysis of AOPP, MDA and hs-CRP was performed. Results Compared with normal control group, significantly higher serum MDA, DHAA/TAA, and AOPP levels were detected in RA patients (P<0.05), but vitamine E showed no significant difference (P<0.05). Linear regression analysis showed that MDA (P<0.01) was positively but AOPP (P>0.05) negatively correlated to hs-CRP. Conclusions Oxidation or reduction products in serum of RA patients increases significantly, which may be an important mechanism for the occurrence and development of RA. Serum AOPP and MDA levels can reflect the oxidation status in RA patients.
出处
《第一军医大学学报》
CSCD
北大核心
2005年第6期749-750,共2页
Journal of First Military Medical University
关键词
类风湿性关节炎
诊断
血清学
氧化还原物/检测
自由基
rheumatoid arthritis
diagnosis, serology
oxidation-reduction/detection
free radicals
