摘要
背景与目的组织因子(tissuefactor,TF)生理功能为凝血过程的启动者。近来发现TF参与多种恶性肿瘤微血管形成的过程。本研究旨在探讨胃癌组织中表达的组织因子与其微血管形成过程之间的关系和临床意义。方法应用免疫组织化学EnVisionTM法,检测了80例胃癌和20例正常胃组织标本中TF、VEGF(血管内皮生长因子)的表达情况和CD34单克隆抗体标记的MVD值(肿瘤微血管密度)。结果胃癌组织中TF和VEGF的阳性表达率分别为65.00%(52/80)和67.50%(54/80),MVD值为36.14±9.94;正常胃组织中TF和VEGF的阳性表达率分别为5.00%(1/20)和5.00%(1/20),MVD值为12.10±3.27。胃癌组织中TF的表达与VEGF的表达和MVD值之间均存在相关性(P<0.05),组织中TF表达越高,相应VEGF的表达越高,MVD值也越大。TF与患者的总生存期、TNM分期和肝脏转移状况有关。结论胃癌组织中异常表达增高的TF与胃癌微血管形成过程和患者预后有关。
BACKGROUND & OBJECTIVE: Tissue factor (TF), the primary physiologic initiator of coagulation cascade, is involved in the process of angiogenesis of various malignancies. This study was designed to investigate the correlation of TF expression to angiogenesis of gastric carcinoma, and to study its clinical significance. METHODS: The expression of TF and vascular endothelial growth factor (VEGF) in 80 specimens of gastric carcinoma and 20 specimens of normal gastric tissue was detected by EnVisionTM immunohistochemistry. Tumor microvessel density (MVD) was evaluated using anti-CD34 antibody as an endothelial marker with the same technique as well. RESULTS: Positive rates of TF and VEGF, and the mean value of MVD were significantly higher in gastric carcinoma than in normal gastric tissue (65.00% vs. 5.00%, P<0.01; 67.50% vs. 5.00%, P<0.05; and 36.14±9.94 vs. 12.10±3.27, P<0.05). TF expression was positively correlated with VEGF expression and MVD value (P<0.05). TF expression was correlated to the overall survival times of patients, TNM stage, and liver metastasis (P<0.05). CONCLUSION: Overexpression of TF relates with angiogenesis and prognosis of gastric carcinoma.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2005年第7期880-884,共5页
Chinese Journal of Cancer
基金
甘肃省自然科学基金(No.S021-A25-069-Y)~~
关键词
胃肿瘤
组织因子
血管内皮生长因子
基因表达
微血管密度
免疫组
织化学
Gastric neoplasms
Tissue factor
Vascular endothelial growth factor
Gene expression
Microvessel density
Immunohistochemistry
