摘要
目的探讨结直肠腺癌组织中淋巴管形成的临床病理意义。方法应用单克隆抗体D2-40采用免疫组化EnVision法检测94例结直肠腺癌周围和中心区以及正常结直肠组织中淋巴管密度(LVD),分析其与结直肠腺癌分化程度、浸润深度、淋巴管侵犯和淋巴结转移的关系。结果肿瘤周围区LVD明显高于中心区和正常组织(P<0.01)。肿瘤周围区LVD与淋巴管侵犯和淋巴结转移密切相关(P<0.01),而与肿瘤的分化程度和浸润深度无关(P>0.05),肿瘤中心区LVD与肿瘤分化程度、浸润深度、淋巴管侵犯和淋巴结转移均无相关性(P>0.05)。结论结直肠腺癌主要是肿瘤周围区存在着淋巴管形成,检测肿瘤周围淋巴管密度可以预测肿瘤淋巴结转移。
Objective To investigate the clinicopathologieal significance of the lymphangiogenesis in colorectal adenocareinoma. Methods Monoclonal antibody D2-40 was used to detect the lymphatic vessel density（LVD） in the central and surrounding tissues of colorectal adenocarcinoma and normal colorectal tissues by immunohistochemistry EnVision. The correlation of LVD in colorectal adenocarcinoma with differentiation, invasion depth,lymphangiogenesis and lymph node metastasis was analyzed. Results LVD in peri-tumoral tissues was significantly higher than that in intra-tumoral tissues and normal tissues（P 〈0.01 ）. LVD in peri-tumoral tissues was significantly associated with involvement of lymphatic vessel and the lymph node metastasis（ P 〈 0.01 ）, but not with differentiation and invasion depth（ P 〉 0.05）. LVD in intra-tumoral tissues was not correlated with the differentiation,invasion depth,involvement of lymphatic vessel and lymph node metastasis （ P 〉 0.05 ）. Conclusion Lymphangiogensis is mainly located in the peri-tumoral area of colorectal adenocarcinoma. Detection of LVD in the peri-tumoral area can be used for predicting lymph node metastasis.
出处
《山西医科大学学报》
CAS
2009年第8期696-699,共4页
Journal of Shanxi Medical University
关键词
结直肠腺癌
D2-40
淋巴管密度
colorectal adenocarcinoma
D2-40
lymphatic vessel density
