摘要
目的 研究亚砷酸钠(sodiumarsenite ,NaAsO2 )对人皮肤角质形成细胞系(HaCaT)的毒性作用及N 乙酰半胱氨酸(N acetylcysteine ,NAC)对砷毒性的拮抗作用。方法 NaAsO2 单独作用于HaCaT细胞2 4h或用NAC预处理后,加入NaAsO2 继续作用2 4h ,用AlamarBlue还原法检测细胞活力。结果 <1μmol/L的NaAsO2 单独作用于细胞2 4h后,AlamarBlue还原率增高(P <0 . 0 5 ) ,10 μmol/L以上的NaAsO2 则使AlamarBlue还原率显著下降(P <0 . 0 1) ;用NAC预处理2 4h后再加入NaAsO2 ,2 0 μmol/L组AlamarBlue还原率与NaAsO2 单独作用相比显著增加(P<0 .0 5 )。结论 低浓度NaAsO2 刺激细胞增殖,高浓度具有细胞毒性;NAC可减轻砷的细胞毒性,细胞内的还原型谷胱甘肽(GSH)对砷的细胞毒性起保护作用。
Objective To study the cytotoxicity of sodium arsenite(Na AsO_2)on keratinocytes(HaCaT) and the protective effect of N-acetylcystein e(NAC).Methods The HaCaT cells were treated with sodium arsenite alone or pre-treated with NAC for 24 hours,followed by the addition of sodium arsenite.AlarmarBlue assay was used to detect the proliferation of the cells.Results The reduction rate of AlarmarBlue increased when the HaCaT cells were treated with 0.001?μmol/L-1?μmol/L o f NaAsO_2 alone(P<0.05).When pre-treated with NAC for 24 hours,the reduc tion rate increased significantly in the 20?μmol/L group compared with the gro up treated with 20?μmol/L of NaAsO_2 alone(P<0.05).Conclusion Low levels of NaAsO_2 stimulate the prolifera tion of HaCaT cells while high levels inhibit it.NAC can alleviate the cytotoxic ity of asenic,which indicate that the cellular GSH exert the important protectiv e effects on the cytotoxicity of arsenic.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2005年第5期587-588,共2页
Chinese Journal of Public Health
