摘要
目的研究细胞内谷胱甘肽(GSH)对砷致人永生化角质形成细胞系(HaCaT)毒性的保护作用。方法HaCaT细胞用N-乙酰半胱氨酸(N-acetylcysteine,NAC)和丁硫氨酸亚矾胺(L-buthionine-眼S’R演-sulfoximine,BSO)预处理后,加入亚砷酸钠(sodiumarsenite,NaAsO2)继续作用,用Alamarblue摄入法检测细胞活力,每组4个复孔。结果单独用NaAsO2作用细胞24h后,0.001～10.000μmol/L组Alamarblue还原率增高(P<0.05),大于10.000μmol/L时Alamarblue还原率下降(P<0.01);用NAC预处理24h后再加入NaAsO2,15μmol/L组Alamarblue还原率比NaAsO2单独作用增加(P<0.05);用BSO预处理24h后再加入NaAsO2,15μmol/L组的Alamarblue还原率均下降(P<0.05)。结论低浓度NaAsO2刺激细胞增殖,高浓度具有细胞毒性;NAC可减轻砷的细胞毒性,而BSO则加重其细胞毒性。
Objective To study the effect of cellular glutathione （GSH） in relieving the cytotoxicity induced by arsenic on HaCaT. Methods The HaCaT ceils were pre-treated with N-acetylcysteine （NAC） and L-buthionine-[S＇ R]-sulfoximine （BSO） for 24 h, then treated with sodium arsenite （NaAsO2） by adding it into the medium for 24 h. Alarmarblue assay was used to detect the proliferation of the cells. Results The reduction rate of Alarmarblue increased when the HaCaT cells were treated with 0.001-10.000 μmol/L of NaAsO2 alone （P〈0.05）, when pre-treated with NAC for 24 hours, the reduction rate in the 15 ixmoVL group increased （P〈0.05）, while pre-treated with BSO for 24 hours, the reduction rate decreased markedly （P〈0.05）. Conclusion Low level NaAsO2 stimulates the proliferation of HaCaT cells, but at high levels it shows an inhibitive effect. NAC can relieve the cytotoxicity of arsenic while BSO may aggravate it. The results of the present paper indicate that the intracellular GSH may play an important role in relief of the cytotoxicity induced by arsenic.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2006年第1期28-30,共3页
Journal of Environment and Health
