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叔丁基对苯二酚对亚砷酸钠致细胞毒性和氧化损伤的拮抗作用 被引量：2

Protective effects of tert-butylhydroquinone on sodium arsenite-induced cytotoxicity and oxidative injuries
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摘要 目的探讨叔丁基对苯二酚（tBHQ）对亚砷酸钠（NaAsO：）致细胞毒性和氧化损伤的拮抗作用。方法Chang肝细胞用tBHQ[0（对照）、5、25Ixmol／L]预处理24h，再用5Ixmol／LtBHQ和NaAs02[O（对照）、30、40、50、60μmol／L]共同作用24h，采用刃天青钠（Alamarblue）还原法检测细胞活力，结果用实验组Alamarblue还原率与对照组Alamarblue还原率的相对比值表示；Chang肝细胞用tBHQ[0（对照）、5、25μmol／L]预处理24h，再用5μmol／LtBHQ和NaAs02[0（对照）、40、50μmol／L]共同作用24h，采用荧光探针2’，7＇-二乙酰二氯荧光素（DCFH—DA）检测细胞内活性氧（ROS）的生成，结果用实验组平均荧光强度与对照组平均荧光强度的相对比值表示。结果30、40、50、60μmol／L的NaAsO：暴露能够显著降低细胞活力，而tBHQ预处理（5、25μmol／L）则可明显恢复细胞活力，NaAsO2和tBHQ两因素的主效应及其交互作用均有统计学意义（F值分别为566．57、55．09、14．50，P均〈0．05）；5、25μmol／LtBHQ预处理的30、40、50、60Ixmol／LNaAs02组细胞活力（0．75±0．02、0．70±0．04、0．59±0．03、0．43±0．03和0．75±0．02、0．734-0．03、0．654-0．02、0．50-t-O．02）较相应NaAs02单独作用组（0．70±0．03、0．644-0．03、0．43±0．03、0．33±0．01）显著升高（P均〈0．05），25μmol／LtBHQ预处理的50、60μmol／LNaAsO2组细胞活力高于相应5μmol／LtBHQ预处理组（P均〈0．05）。40、50μmol／L的NaAsO2能显著诱导Chang肝细胞内ROS的产生，而tBHQ预处理（5、25μmol／L）则可使NaAs02诱导产生的细胞内ROS水平显著下降，NaAsO2和tBHQ两因素的主效应及其交互作用均有统计学意义（F值分别为181．78、60．55、4．93，P均〈0．05）；5、25μmol／LtBHQ预处理的40、50μmol／LNaAs02组细胞内ROS水平（1．87±0．09、1．80±0．07和1．36±0．1l、1．44±0．12）较相应NaAs02单独作用组（2．30±0．18、2．184-0．17）显著降低（P均〈0．05），25μmol／LtBHQ预处理的40、50μmol／LNaAs02组细胞内ROS水平低于相应5μmo／LtBI-IQ预处理组（P均〈0．05）。结论tBHQ对NaAsO2诱导的细胞毒性和氧化损伤具有一定的拮抗作用。 Objective To study the protective effects of tert-butylhydroquinone（tBHQ） on sodium arsenite （NaAsO2）-indueed cytotoxicity and oxidative injuries. Methods Chang liver cells were pretreated with tBHQ [0 （control）, 5, 25 μmol/L] for 24 h, and then co-treated with tBHQ（5 μmol/L） together with NaAsO2[0（control）, 30, 40, 50, 60 μmol/L] for another 24 h, and Alamar blue reduction rates were used to evaluate cell viability, the results were expressed as the relative ratio of Alamar blue reduction rates between the experimental group and the control group. On the other hand, Chang liver cells were pretreated with tBHQ[0（control）, 5, 25 μmol/L] for 24 h, and then co-treated with tBHQ（5 μmol/L） together with NaAsO2[0（control）, 40, 50 μmol/L] for another 24 h, and the levels of cellular reactive oxygen species （ROS） were detected by staining cells with 2＇ ,7＇-dichlorofluorescin diaeetate（DCFH-DA）, the results were expressed as the relative ratio of mean fluorescence intensity between the experimental group and the control group. Results Cell viability decreased dramatically by treatment with NaAsO2（30, 40, 50, 60 txmolfL）, while relieved to some extent by pretreatment with 5, 25μmol/L tBHQ, the main effects of NaAsO2 and tBHQ, as well as their interaction were all statistically significant（F = 566.57, 55.09, 14.50, all P 〈 0.05） ; the cell viability of NaAsO2（30, 40, 50, 60 μmol/L） pretreated with tBHQ（5, 25 μmo]/L） were 0.75 ± 0.02, 0.70 ± 0.04, 0.59 ± 0.03, 0.43 ± 0.03 and 0.75 ± 0.02, 0.73 ± 0.03, 0.65 ± 0.02, 0.50 ± 0.02, respectively, all significantly higher than corresponding NaAsO2 alone groups（0.70 ± 0.03, 0.64 ± 0.03, 0.43 ± 0.03, 0.33 ± 0.01, all P 〈 0.05）, the cell viability of NaAsO2（50, 60 μmol/L） pretreated with 25 μmol/L tBHQ was higher than corresponding 5 Ixmol/L tBHQ pretreatment groups （all P 〈 0.05）. On the other hand, 40, 50 I±mol/L of NaAsO2 significantly induced hepatocellular ROS generation, while tBHQ （5, 25 μmol/L） pretreatment significantly decreased NaAsO2-induced intracellular ROS levels, the main effects of NaAsO2 and tBHQ, as well as their interaction were all statistically significant（F = 181.78, 60.55, 4.93, all P 〈 0.05）; the ROS levels of NaAsO2 （40, 50 I±mol/L） pretreated with tBHQ（5, 25 ±mol/L） were 1.87 ± 0.09, 1.80 ± 0.07 and 1.36 ± 0.11, 1.44 ± 0.12, all significantly decreased than corresponding NaAsO2 alone groups （2.30 ± 0.18, 2.18 ± O. 17, all P 〈 0.05 ）, the ROS levels of NaAsO2（40, 50 μmol/L） pretreated with 25 μmol/L tBHQ decreased than corresponding 5 μmol/L tBHQ pretreatment groups （all P 〈 0.05）. Conclusion tBHQ has a certain antagonism on arsenic induced cytotoxicity and oxidative injuries.
作者 李冰 李昕 朱博 张新玉 邢晓越 刘丹 王欣 孙贵范
机构地区 中国医科大学公共卫生学院劳动卫生学教研室
出处 《中国地方病学杂志》 CAS CSCD 北大核心 2011年第5期489-492,共4页 Chinese Jouranl of Endemiology
基金 基金项目：国家自然科学基金（30600510、81072243） 辽宁省教育厅高等学校科研项目计划（L2010704）
关键词 亚砷酸盐类 叔丁基对苯二酚 活性氧 Arsenites Tert-butylhydroquinone Reactive oxygen species
分类号 R [医药卫生]
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参考文献11

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共引文献1

同被引文献24

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中国地方病学杂志
2011年 第5期

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