摘要
免疫酶标法用于观察肺组织中胶原的分布。显微分光光度法用于研究大鼠与矽肺病人的纤维化组织中Ⅰ型与Ⅲ型胶原的相对含量。染色中应用大鼠及人的抗Ⅰ型与Ⅲ型胶原多克隆抗体。Ⅰ型胶原分布于正常肺泡间隔及间质中。Ⅲ型胶原也在相同的部位存在。在由石英所致的大鼠纤维化肺组织中,Ⅰ型与Ⅲ型胶原在极度扩展的间质中聚集。Ⅲ型胶原是早期纤维化肺中主要增生的胶原。Ⅰ型胶原是纤维化后期主要增生的胶原。在矽肺病人肺组织中也见到同样的现象。染电焊烟尘的大鼠肺,直至180天以前,Ⅲ型胶原为主要积聚的胶原,而在180天以后,Ⅰ型胶原的增加才占优势。
ELISA method was used to stain the lung tissues for the observation of collagen distrlbation and microscopic spectrophotometric method was used to study the relative amount of Type Ⅰ and Ⅲ collagen in fibrotic lungs of rats and silicotic patients. Polyclonal specific antibodies of rat Type Ⅰ and Ⅲ collagen as well as those of human collagen were used in this staining. Type Ⅰ collagen was found in alveolar septa and interstitium and Type Ⅲ collagen shared the same feature. In fibrotic Lung induced bysilica, we observed that Type Ⅰ and Ⅲ collagen accumulated heavily in the expandedinterstitium. Type Ⅲ collagen appeared to be the predominated collagen in the early stage of fibrosis, whereas Type Ⅰ collagen was the main collagen found in the later stage. Similar results were obtained in silicotic patients' lung using the same technique.In lungs of rats instilled with welding dust, Type Ⅲ collagen was found accu mulated predominantly until 180 days after instillation, while Type Ⅰ aollagen increased predominaotly after 180 days.
出处
《卫生研究》
CAS
CSCD
北大核心
1989年第2期1-3,共3页
Journal of Hygiene Research
关键词
矽肺
Ⅰ型胶原
肺组织
Ⅲ型胶原
Type Ⅰ and Type Ⅲ Lung Collagen,Silica,Welding fume dust, ELISA Staining of collagen
