摘要
使用铜蓝蛋白ELISA法测定了矽肺大鼠及汉防己甲素(汉甲)、克矽平、磷酸羟基喹哌(抗矽Ⅰ号)及柠檬酸铝(柠铝)治疗的矽肺大鼠血清铜蓝蛋白(Cp)含量,并与微量法测定结果进行了比较。两种方法测定结果十分相近。ELISA法对血清存放时间要求较宽,Cp的微量测定法简便准确,但要求血清存放时间短。
Ceruloplasmin (Cp) microanalysis method is simple and accurate. However, the samples must be measured within 24 h. Using ELISA method, we analyzed serum Cp levels in 234 silicotic rats before and after treated by Tetrandine (TT), Polyvinylpyridine-N-Oxide (PVNO), Hydroxypiperaquinoline phosphate and aluminium citrate. The ELISA results consist with the results by microanalysis method. Since ELISA is based on immunoreaction, instead of enzyme reaction for microanalysis method, the sera can be examined by ELISA after frozen storage. The authors recommend the ELISA method for silicosis research.
出处
《卫生研究》
CAS
CSCD
北大核心
1996年第1期4-5,共2页
Journal of Hygiene Research
基金
国家"八五"攻关项目
