摘要
本文采用近年来发展的单细胞凝胶电泳试验检测了二氯甲烷、三氯甲烷、四氯化碳及一氯乙酸和三氯乙酸对Ｖ７９细胞的ＤＮＡ损伤作用。结果显示：五种受试物均可引起体外培养的Ｖ７９细胞ＤＮＡ损伤，但其作用强度差异很大。其顺序为四氯化碳＞三氯甲烷＞二氯甲烷＞一氯乙酸＞三氯乙酸。该结果还提示：五种氯化有机物对Ｖ７９细胞的毒性及ＤＮＡ损伤作用的大小顺序是一致的，氯化烷基类化合物的细胞毒性及ＤＮＡ损伤作用均大于氯乙酸类，在氯化烷基类化合物中。
In this paper, the DNA damage effects of carbon tetrachlororide, trichloromethane, dichloromethane, chloroacetic acid and trichloroacetic acid were studied using the single cell gel electrophoresis technique(comet assay) in V 79 cells cultured in vitro. The results showed that all test substances, except trichloroacetic acid, exhibited a clear and reproducible linear dose dependent increase in DNA migrtion. The DNA breakage capacity and the cytotoxicity of this chemicals have the same order: carbon tetrachlororide>trichloromethane>dichloromethane>chloroacetic acid>trichloroacetic acid. The data also suggested that DNA breakage capacity and the cytotoxicity of the chemicals of chlorinated alky1 were stronger than that of the kind of chloroacetic acid. Among the chemicals of chlorinated alky1, the DNA breakage capacity increased with increasing in the number of chlorine substitution.
出处
《癌变·畸变·突变》
CAS
CSCD
1999年第1期5-8,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金
关键词
氯化有机物
单细胞凝胶电泳
DNA损伤
V79细胞
Chlorinated organic compound
Single cell gel electrophoresis
DNA damage
V 79 cell
