摘要
在ｐＨ值为４．２的ＢＲ缓冲溶液中，氯氮（利眠宁）溶液经紫外光照射后，光化学反应产物具有荧光性质，λ_（ｅｘ）为２６５及３７０ｎｍ，λ_（ｅｍ）为４４２ｎｍ。氯氮浓度在０～３．００μｇ／ｍｌ范围内与荧光强度呈良好的线性关系。借此建立了氯氮的光化学－荧光测定新方法。其检出限为１６ｎｇ／ｍｌ，相对标准偏差为１．３％，可用于片剂中氯氮 含量的测定。
In pH 4. 2 buffer solution chlordiazepoxidum leads to a fluorescent photochemical reaction product under the irradiation of ultraviolet light. The excitation wevelength of the fluorescent product is 265 and 370nm and the emission wevelength is 442nm. The fluorescence intensity is linearly increased with the concentration of chlordiazepoxidum in the range of 0￣3. 00μg/ml. On this basis, a photochemical- fluorimetric method was developed for the determination of chlordiazepoxidum. The detection limit and relative standard deviation were 16ng/ml and 1. 3%, respectively. This method has been used for the determination of chlordiazepoxidum in the tablets.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1994年第1期26-28,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
氯氮卓
光化学荧光法
chlordiazepoxidum
photochemical- fluorimetric method
