摘要
目的 :探讨补髓生血颗粒对慢性再生障碍性贫血 (CAA)患者CD34 +细胞及CD34 +C -myc细胞表达影响及疗效机理。方法 :应用免疫组化技术和双染法标记 ,检测 36例CAA患者治疗前后骨髓CD34 +细胞及CD34 +C -myc细胞表达水平。结果 :CAA患者CD34 +细胞表达疗前和疗后均低于正常组 (P <0 .0 5 ) ,疗后与疗前相比差异显著 (P <0 .0 5 ) ;CD34 +C -myc细胞表达疗前和疗后均高于正常组 (P <0 .0 5 ) ,疗后与疗前相比差异显著 (P <0 .0 5 ) ;且实验组疗效优于对照组 (P <0 .0 5 )。结论 :补髓生血颗粒作用机制之一可能是通过降低C -myc蛋白表达 ,从而提高CAA患者骨髓造血干细胞水平的。
Objective:To observe the effects and the mechanism of the soluble granules of Busui Shengxue(补髓生血冲剂)to Chronic Aplastic Anemia(CAA).Methods:Doublelabel immunohistochemical method to observe the level of CD + 34 and CD 34 + C-MYC BEFORE AND AFTER TREATMENT IN 36 CAA patients.Result:CD + 34 of CAA patients showed lower level before and after treatment than normal groups ( P< 0.05 ),CD + 34 showed higher level after treatment than before( P< 0.05 );CD 34 +C-myc of CAA patients showed higher level before and after treatmen than normal groups( P< 0.05 ),CD 34 +C-myc showed lower leve after treatment than before ( P< 0.05 );Experimental groups showed better effect than opposite groups( P< 0.05 ).Conclusion:One of the mechanism of the soluble granules of Busui Shengxue(补髓生血冲剂)probably increased the level of CAA patients'bone marrow hemopoietic stem cell by decreasing the level of C-myc. Author's address:The 210 Hospital of Dalian,Dalian 163515,China
出处
《中医药信息》
2005年第2期54-55,共2页
Information on Traditional Chinese Medicine
基金
国家自然科学基金资助项目 (30 2 71 62 1 )
