摘要
探讨人参皂甙（ＧＳ）对人骨髓ＣＤ３４＋造血干细胞的增殖作用。方法：采给Ｄｙｎａｌ Ｍ４５０ ＣＤ３４＋免疫磁珠分离法阳性选择获取高纯度的人骨髓ＣＳ３４＋造血干细胞，应用多向祖细胞（ＣＦＵ－Ｍｉｘ）体外培养技术，观察ＣＤ３４＋造血干细胞对ＧＳ的敏感性。结果：１．经Ｄｙｎａｌ Ｍ－４５０ ＣＤ３４＋免疫磁珠分离获得骨３髓 ＣＤ３４＋细胞占骨髓单个核细胞（ ＭＮＣ）的 ０． ５２～１．３０％；ＣＤ３４＋细胞的存活率达９４．０～９８．２％。 ２最佳浓度的ＧＳ使ＣＤ３４＋细胞的ＣＦＵ－Ｍｉｘ集落形成率提高５４．４％（Ｐ＜０．０１），而高浓度时表现为抑制。结论：ＧＳ不但作用于人骨髓造血祖细胞，而已对ＣＤ３４＋造血干细胞也具有明显的刺激增殖作用。
To investigate the proliferation effects of Ginsenosides (GS) on CD34positive hematopoietic stem cells from human bone marrow Methods: Highly Purified CD34+ hematopoietic stem cells were isolated from human bone marrow by using a Dynal CD34 Progenitor CellSelection System(Dynal Norway). The potentiated effects of GS on CD34+ hematopoietic stem cells were observed with an agarsemi - solid culture of CFU - Mix colony assay. Results: The number of CD34+ hematopoietic stem cells after being purified from Dynal beads system was 0. 52%- 1. 30%out of mononuclear cells of bone marrow Viable cells were 94. 0% - 98. 2%out of CD34positive cells GS could stimulate the proliferation of CD34+ hemetopoietic stem cells in colony culture assay, the numbers of CFU - Mix in the presence of GS 25and50mg/L were increased by 49. 3± 4. 9% and54. 4±6. 6% overthose of non - GS control respectively(P < 0. 01, each) Conclusion: The results above indicated that GS couldnot only stimulate the proliferation of progenitor cells, but also signiflcanty enhance the growgh ofCD34+ hematopoietic stem cells.
出处
《浙江临床医学》
2000年第2期75-77,共3页
Zhejiang Clinical Medical Journal
关键词
人参总皂甙
造血祖细胞
造血细胞
免疫磁珠
ginsenosides(GS) CD_(34)^+ hematopoietic stem cells proliferation Dynabeads.
