摘要
通过设计特殊摇瓶,用亚硫酸钠法测出摇瓶的体积氧传递系数和氧透过纱布层的通透率。以氧电极测其内外氧的分压降后,可以算出摇瓶表观体积氧传递系数(k_La)_(app)及真实体积氧传递系数k_La,并进一步求出氧通透率。由实验得出:氧分压降低6.1％,氧传递系数增加一倍;在37℃、220r/min、500ml摇瓶(内盛液50ml)8层纱布的氧通透率P_m=43.7mol/m^2·h·atm;并且关联出摇瓶容积V、装液量V_L、转速n、摄氏温度t之间的模型式: (k_La)_(app)=1.84×10^(-7)[t]^(1.8497)·[n]^(2.3906)·[V_L/V]^(-0.6360) (k_La)=2.02×10^(-7)[t]^(1.8525)·[n]^(2.3941)·[V_L/V]^(-0.6370)
The Oxygen permeability passing through the gauze layer and the actual and apparent volumetric oxygen transfer rate-k1a and (k1a)app were determined in the special designed Erlenmeyer flasks which have an oxygen electrode inserted in the middle part of flasks. The oxygen transfer rate was determined by sulfite oxidation method. As the result, the oxygen permeability passing throu'gh 8 layers of gauze, at 37℃ , 220 r/min, with 50ml sulfite solution in 500ml flask was 43. 7mol/m2 ?h ?atm; as the oxygen partial pressure decreasing 6. 1% , the value of kLa increased by 100% ; the relationship be-teen rotational speed of shaker, n (r/min); volume of flask, V (ml); liquid content in flask, VL (ml); temp, of shaker, t (℃) and kLa as well as (kLa)app (1/h) were
出处
《生物工程学报》
CAS
CSCD
北大核心
1994年第2期114-117,共4页
Chinese Journal of Biotechnology
基金
国家教委博士点基金
关键词
摇瓶
体积氧传递
系数
氧通透率
Shaken flask, volunetric oxygen transfer coefficient, oxygen perjneability, engineering scale - up
