摘要
目的 :探讨带血管蒂周围神经促进胚胎脊髓修复成鼠脊髓损伤的能力。方法 :成鼠胸段脊髓损伤后 ,分别移植带血管蒂正中神经 (VPN组 )、孕 14天胚胎脊髓 (FSC组 )、带血管蒂正中神经加胚胎脊髓 (V +F组 )。术后 8周行组织学及电生理检查。结果 :V +F组移植神经与脊髓连接紧密 ,有较多新生轴突长入 ,雪旺氏细胞大量存活和增殖 ,神经丝蛋白、10 0 %饱和硫蛋白阳性反应均明显高于VPN组 (P <0 0 1) ;胚胎移植物与受体融合佳 ,体积增长速度、神经纤维和神经元数目显著高于FSC组 (P <0 0 1) ,大部分细胞分化较好 ,突触较成熟 ;SEP检查示P1、N1波潜伏期显著缩短 (P <0 0 1)。结论 :带血管蒂周围神经与胚胎脊髓联合移植 ,对FSC的生长发育。
Objective:To observe the repairment effects of vascular pediceled nervous tissue on adult rat′s spinal cord injury.Method:A 5mm cord defect of the left lateral column was made at T 1—3 vertebral level,and then the defect was grafted randomly using vascularized peripheral nerve (Group VPN),embryonic day 14 fetal spinal cord(Group FSC) or vascular pediceled peripheral nerve and fetal spinal cord(Group V+F).8 weeks after surgery, the survival,differentiation of the grafts,and the ability of repaired host spinal cord were assessed.Result:In VPN FSC co graft group, peripheral nerve segment was extensively vascularized and well innervated,plenty of Schwann cells survived and proliferated.In this group,anti NF,S 100 positives fibers were significantly greater than group VPN ( P <0 01).The fetal implant was intimately fused with the host CNS parenchyma,the volume,nerve fibers and neurons density of the fetal implant were significantly higher than Group FSC( P <0 01).The majority of neurons in this group were well differentiated,well developed and synapses were scattered throughout the neuropil.SEP assessments showed that the latency of P 1,N 1 wave was significantly decreased in this group ( P <0 01).Conclusion:The vascular pediceled peripheral nerve could prompt the development of fetal implant and support the regeneration of central injured axons to certain degrees. Author′s address\ Dept. of Orthopedics, Daping Hospital, The Third Military Medical University, Chongqing, 400042
出处
《中国康复医学杂志》
CAS
CSCD
2001年第1期15-18,共4页
Chinese Journal of Rehabilitation Medicine
