摘要
在建立带血管神经（ＶＮ）干移植修复脊髓损伤的基础上，对自体ＶＮ与游离神经（ＦＮ）移植修复脊髓损伤的研究进行了比较。结果表明：ＶＮ与脊髓连接处无明显空洞反应，瘢痕组织少，大量神经纤维长入移植神经内，ＶＮ组在脑干、脊髓及神经根背节有２９２２±７５４．３ＨＲＰ神经元，而ＦＮ组仅只有２０±６．５ＨＲＰ神经元（Ｐ值＜０．０１）。有髓轴突的数目、直径及截面积ＶＮ组非常明显地大于ＦＮ组（Ｐ值＜０．０１）认为：带血管神经移植后，改善移植物及其周围组织的血液供应；保证雪旺氏细胞成活，产生多种高浓度神经营养因子；促使成纤维细胞释放大剂量载脂蛋白Ｄ，有利于髓鞘的形成。
On the basis of establishing a new adult rat model of vascularized nerve trunk autografted in spinal cord, we compared it with non-vascularized nerve trunk autografts. The grafts in the vascularized nerve group(VNG) contained by many new fibres; In the free nerve group (FNG), however, only a few new axons regenerated into the graft. The origins and terminations of axons in the graft were studied by retrograde neuronal labelling with horseradish peroxidase (HRP). The results showed that axons in VNG origated from central nervous system neurons with their cell bodies from brain stem to sacral segments of the spinal cord and that others arose from dorsal root ganglia at the level of the grafts and at least 16 sgements distal to them. Combining studies of electron microscopy, electophysiology, silver and H&E stain, these results demonstrated that peripheral nerve trunk autografted in spinal cord in VNG was regenerating much better than that in FNG. The number, diameter and area of the new myelinated axons in VNG were significantly greater than that in FNG( P<0. 001). This findings indicated that CNS is able to regenerate better in vascularized nerve autoografted in the spinal cord.
出处
《中华神经外科杂志》
CSCD
北大核心
1996年第3期160-163,共4页
Chinese Journal of Neurosurgery
关键词
带血管神经
移植
脊髓损伤
Vascularized nerve Transplantation Spinal cord injury
