摘要
背景:脊髓损伤后以游离周围神经片断(雪旺氏细胞)与胚胎脊髓联合移植能协同诱发宿主再生潜能,而带血管蒂周围神经移植后有更多的雪旺氏细胞存活,但其与胚胎脊髓协同作用的研究较少。目的:探讨不同神经组织移植修复成鼠急性脊髓损伤的能力。设计:单盲随机对照实验。地点和对象:实验在解放军第三军医大学大坪医院全军战创伤中心骨创伤科完成,研究对象为150只4月龄雌性Wistar属成年大鼠,由解放军第三军医大学实验动物中心提供。干预:成鼠胸髓损伤后,分别移植游离正中神经(FPN组)、带血管蒂正中神经(VPN组)、孕14天胚胎脊髓(FSC组)、游离正中神经加胚胎脊髓(P+F组)、带血管蒂正中神经加胚胎脊髓(V+F组)。主要观察指标:术后8周行神经解剖及电生理检查。结果:V+F组再生轴突和存活雪旺氏细胞数目、胚胎脊髓体积增长速度和神经元密度显著高于对照组(P<0.01),细胞分化较好,突触较成熟。SEP的P1,N1波潜伏期显著缩短(P<0.01)。结论:带血管蒂周围神经与胚胎脊髓联合移植,在解剖和电生理上均优于其他组织,对FSC的生长发育及损伤神经元的再生能力均有一定的促进作用。
BACKGROUND:United transplantation of free peripheral nervous fragment(Schwann cell,SC) and fetal spinal cord(FSC) after spinal cord injury(SCI) can synergisti cally induce the regeneration potential in host; and furthermore, more SC will s urvive after the transplantation of vascularized peripheral nerves(VPN),however, few researches about its synergistic reactions with fetal spinal cord.OBJECTIVE:To discuss the repair abilities of different nervous tissue transpla ntation in acute SCI in adult rat. DESIGN:A single-blind randomized controlled study was performed.SETTINGS and PARTICIPANTS:The study was completed in the Department of Orthopa edics and Traumatology,Center for War Injury of Daping Hospital,Third Military M edical University.Totally 150 4-month-old female Wistar adult rats provided by Center for Experimental Animals of Third Military Medical University.INTERVENTIONS:After injury in thoracic spinal cord,these adult rats were trans planted respectively with free median nerve(FPN group),vascularized median nerve (VPN group),14-day pregnancy fetal spinal cord(FSC group), free median nerve an d fetal spinal cord(P+F group), and vascularized median nerve and fetal spinal cord(V+F group).MAIN OUTCOME MEASURES:Nervous anatomy and electrophysiological examination wer e examined after 8-week surgery.RESULTS:The numbers of regenerated axons and survived SCs,growing speed of fet al spinal cord volume as well as the density of neurons in V+F group were signi ficantly higher than that of control group(P< 0.01) with better differentiation and more mature synapse.The latencies of P1 and N1 wave of somatosensory evoked potentials(SEP) examination were significantly shortened(P< 0.01).CONCLUSION:The united transplantation of vascularized peripheral nerve and fet al spinal cord is better than other tissues on anatomy and electrophysiology, wh ich has certain promoting reactions on growth and development of FSC and the rep air ability of injured neuron.
出处
《中国临床康复》
CSCD
2004年第11期2127-2129,共3页
Chinese Journal of Clinical Rehabilitation
