摘要
目的 用ACHN肾癌细胞株,建立人肾癌裸小鼠异位移植模型。方法 将ACHN人肾癌细胞株2. 0×10 /0. 2ml接种于裸小鼠背部皮下,观察移植肿瘤的生长状况、移植成功率、肺转移率及移植瘤病理形态,检测肿瘤细胞的增殖与凋亡。结果 异位移植成功率为 75%,肺转移率 58. 3%,肿瘤细胞增殖指数(PI)为 54. 84±11. 39,凋亡指数(AI)为 1. 75±0. 83,AI/PI与肿瘤体积呈负相关 (r=-0. 8332,P<0. 05)。皮下瘤病理形态与肾腺癌相似。结论 人ACHN肾癌裸鼠异位移植瘤模型可作为肾癌及其转移的研究工具。
Objective To develop xenogenous implant model of human ACHN renal cell carcinoma in nude mice. Methods ACHN renal cell carcinoma cells (2.0 10 6/0.2ml) were inoculated subcutaneously to nude mice back. The local tumor growth characteristic, tumor-take rate, lung metastasis rate, pathologic characteristic of tumor were examined. After 31 days, the mice were sacrificed. Cell proliferation index and apoptotic index were detected. Results Tumor-take rate and lung metastases rate were observed in 75% and 58.3% of nude mice respectively. Subcutaneous tumor cell proliferation index (PI) and apoptotic index (AI) were 54.84±11.39 and 1.75±0.83 respectively. Statistical analysis reveals an inverse correlation between the AI/PI and the tumors volume (r=-0.8332,P<0.05). Pathologic characristics of xenogrft tumor was similar with human renal adeneocarcinoma. Conclusion This tumor model might be a useful tool in the researches on growth and metastasis of human renal cell carcinoma.
出处
《现代肿瘤医学》
CAS
2005年第1期32-33,共2页
Journal of Modern Oncology
关键词
肾癌
裸鼠
动物实验
异位移植
renal cell carcinoma
animal experiment
xenogenous implant
nude mice
