摘要
目的：观察小干扰RNA（siRNA）沉默FBXO22基因对人肾癌细胞株（786－O、ACHN）细胞迁移、侵袭能力的影响。方法分别用siRNA－Ctrl和siRNA－FBXO22小干扰RNA转染人肾癌细胞株786－O和ACHN细胞；Western blot实验检测FBXO22蛋白表达量，CCK－8实验检测FBXO22基因对肾癌细胞增殖的影响，Transwell细胞迁移实验和侵袭实验检测沉默FBXO22基因后对786－O和ACHN肾癌细胞迁移能力和细胞侵袭能力的影响，明胶酶谱实验检测细胞分泌基质金属蛋白酶（ MMPs）的能力。结果转染FBXO22 siRNA后肾癌786－O及ACHN细胞的FBXO22表达下调，迁移和侵袭能力提高，分泌MMP－9的能力升高，但对肾癌细胞增殖无影响。结论沉默FBXO22基因后，可以增加MMP－9的分泌，从而激活MMP－9信号通路增强肾癌786－O细胞及ACHN细胞的迁移和侵袭的能力。
Objective To investigate the effects of FBXO 22 silencing by small interfering RNA （ siRNA） on the mi-gration and invasion of human renal carcinoma 786-O cells and ACHN cells .Methods The siRNA-control and siR-NA-FBXO22 fragments were transfected into 786-O and ACHN cells .The level of FBXO22 was detected by Western blotting.The effects of FBXO22 silencing on cell proliferation were examined by CCK 8 assay.The effects of FBXO22 si-lencing on the migration and invasion of 786-O and ACHN cells were investigated by Transwell assay .The levels of ma-trix metalloproteinase （ MMPs） were detected by gelatin zymography .Results FBXO22 siRNA could down -regulated the level of FBXO22 in 786-O and ACHN cells, but stimulate the cells′capacities to migration and invasion and en-hance the secretion of MMP -2, without no influence on renal cancer cells .Conclusion FBXO22 silencing can stimu-late the secretion of MMP-9, which would then activate the migration and invasion of renal carcinoma cells .
出处
《徐州医学院学报》
CAS
2016年第3期141-145,共5页
Acta Academiae Medicinae Xuzhou
基金
基金项目：国家自然科学基金面上项目（81472663）
关键词
FBXO22
肾癌
迁移
侵袭
FBX022
renal cell carcinoma
migration
invasion
