摘要
目的 利用大鼠建立慢性青光眼模型。方法 选用成年Wistar大鼠,左眼为实验眼,右眼为对照眼,找到实验眼的4条表层巩膜静脉,烧灼其中3条。定期测量和记录双眼眼压,观察眼前节情况;测量和比较双眼角膜水平直径大小。分别于烧灼后2、4、6个月时摘除双侧眼球。观察大鼠视网膜及视盘的组织形态学改变、双眼视网膜厚度和视网膜神经节细胞数目变化。结果 (1)一次性实验操作可使眼压持续升高至少6个月。(2)眼前节无明显并发症。(3)实验期间,角膜水平直径大小无改变。(4)视网膜厚度明显变薄,视网膜神经节细胞数明显减少,视盘凹陷逐渐扩大和加深。 结论 通过烧灼大鼠表层巩膜静脉可获得长期眼压中度升高的慢性青光眼模型。
Objective To establish a chronic glaucoma model in Wistar rats. Methods Chronic glaucoma model was created in 21 Wistar rats by cauterizing episcleral vessels. Three episcleral vessels of the left eye of each rat were cauterized, and the right eye was used as contol. The intraocular pressure (IOP) in both model and control eyes was measured and recorded regularly. Eyes of experimental rat were enucleated in 2 ,4 and 6 months after operation to observe the changes of optic disk and retina,and numbers of retinal ganglion cells in central and peripheral retina were counted. Results The average IOP was (35. 0±1. 6) mmHg in model eyes with an approximately 1. 8-fold more than that of control eyes (19. 4±1. 1) mmHg, and elevated IOP persisted for at least 6 months after operation. No any complication in the anterior segment was seen in model eyes. Retinal thickness became thinner with an about 46% loss of retinal ganglion cells in the peripheral retina,and 36% in the central retina. C/D of model eye was over 0. 7 after 6 months. Conclusion Establishment of chronic glaucoma model by unilaterally cauterizing the episcleral vessels in Wistar rats offers a reproducible and quantitative way for glaucomatous pharmacology study, observation of damage of optic nerve and neuroprotection as well as treatment of the optic neuropathy associated with glaucoma.
出处
《眼科研究》
CSCD
北大核心
2004年第6期598-600,共3页
Chinese Ophthalmic Research
关键词
青光眼
模型
大鼠
视神经
glaucoma
animal model
rat
optic nerve
