摘要
目的:研究慢性高眼压动物模型的制作方法。方法:选择20只健康Wistar大鼠,雌雄不限,随机分为高眼压组(10只)和sham对照(假手术)组(10只)。高眼压组采用电凝鼠巩膜表面至少三组静脉及角膜缘周围血管,减少房水静脉回流升高眼压,而sham对照组只剪开球结膜,未进行巩膜表面静脉以及角膜缘周围血管电凝。分别于术后1week(w)、2w、3w、4w及8w测大鼠双眼压,结果进行统计学分析。结果:高眼压组右眼术后眼压明显升高。术后1周眼压:(30.35±4.98)mmHg(1kPa=7.5mmHg),术后1至8周间眼压基本稳定。术后各时间点高眼压组右眼眼压与术前、左眼及sham对照组右、左眼间比较,差异有统计学意义(P<0.001)。结论:采用电凝鼠巩膜表面至少三组静脉及角膜缘周围血管,减少房水静脉回流能成功复制慢性高眼压动物模型。
Objective： To study the duplicating method of the chronic intraocular hypertension model. Methods： Twenty Wistar rats were randomly divided into ocular hypertension and Sham control groups. Intraocular pressure （lOP） was raised by electmeoagulating at least 3 episeleral and limbal veins on the right eye of each rat in ocular hypertension group and its eontralateral eye was used as control. At 1 week, 2, 3, 4 and 8 weeks after raising lOP, lOP were measured. Results： Electrocoagulation treatment caused a significant lOP increase of the right eyes in ocular hypertension over the baseline, eontralateral eyes, sham control eyes （ P 〈 0. 001 ）. At 1 week, lOP was （30.12 ± 5.18 ） mmHg （ 1kPa = 7.5mmHg）, and stabilized there after to 8 weeks. Conclusion： The. chronic intraoeular hypertension model eould be successfully duplicated by eleetmcoagulating at least 3 episeleral and limbal veins.
出处
《华西医学》
CAS
2005年第4期699-701,共3页
West China Medical Journal
关键词
青光眼
高眼压
动物模型
Glaucoma
intraocular hypertension
animal model
