摘要
目的:建立一种用高效液相色谱法分离检测阿司匹林和游离水杨酸的方法。方法:以HYPERSIL,BDS,C18(5μm,4.6×250mm)为固定相;乙腈-甲醇-0.01mol·L^(-1)磷酸二氢钾-三乙胺(10:50:40:0.11,磷酸调pH3.3～3.4)为流动相;检测波长280nm。结果:阿司匹林在2.0～10μg浓度范围内。水杨酸在0.06～0.3μg浓度范围内与峰面积呈线性关系。方法的平均回收率,阿司匹林为100.1％,RSD=0.65％(n=5);水杨酸为98.2％,RSD=1.28％(n=5)。结论:方法不需提取,溶解后直接进样,方便快捷,可作为质控方法。
Object: A HPLC method was established for separating and determining Aspirin and free Salicylic Acid limitation in Aspirin Tablets. Methods: Chromatographic Column is HYPERSIL, BDS, C18(5μ,4.6×250mm). The mobile phase is consisted of acetonitrile-methanol-0.01mol·mL^(-1)potassium dihydrogen phosphate-triethylamine(10: 50: 40: 0.11, adjust pH3.3～3.4 by phosphoric acid)and the detection wavelength is 280nm. Result: The linear ranges were 2.0～10μg for Aspirin and 0.06～0.3μg Salicylic acid. The calibration curves were A=5595.9c+207014.0, γ=0.9997 for Aspirin. A=17770.53c+6553.8, γ=0.9999 for Salicylic acid. The average recovery of Aspirin and Salicylic acid were 100.1%, RSD=0.65%(n=5), and 98.2%,RSD=1.28%(n=5)respectively. Conclusion: The method is dependable accurate and easy to operation.
出处
《中国药品标准》
CAS
2000年第2期49-51,共3页
Drug Standards of China
