摘要
目的 探讨核转录因子早期生长反应基因 (Egr 1)在矽肺发生发展中的作用。方法 建立大鼠矽肺模型 ,用免疫组化SP法结合图像分析技术观察了大鼠矽肺组织中各种细胞表达Egr 1、转化生长因子 β1(TGF β1)和纤维黏连蛋白 (FN)的动态变化及相互关系。结果 大鼠矽肺组织中肺巨噬细胞、肺泡上皮细胞、支气管上皮细胞、间质细胞的Egr 1表达 (灰度值范围 118.58± 5.65～ 168.52± 5.67)比对照组 (灰度值范围 166.2 3± 5.2 3～ 188.12± 8.3 5)均明显增强 ,且主要定位于胞核 ;这 4种细胞表达TGF β1(灰度值范围 12 3 .49± 5.65～ 170 .2 4± 3 .56)比对照组 (灰度值范围 166.53± 6.2 5～ 198.56± 4.53 )也明显增强 ,且定位于胞浆。在大鼠支气管上皮细胞、肺巨噬细胞、肺泡上皮细胞中FN表达较弱 (灰度值范围150 .3 2± 6.54～ 2 0 1.54± 7.3 8) ,但间质细胞中呈高表达 (灰度值范围 12 1.43± 5.65～ 167.55± 6.3 5)。实验第 1～ 2 8天 ,TGF β1和Egr 1在大鼠支气管上皮细胞、肺巨噬细胞、肺泡上皮细胞和间质细胞表达变化基本同步 ,二者呈正相关 (r =0 .61,P <0 .0 1) ;而FN在上述细胞中表达和Egr 1未见相关性 ;FN与TGF β1在间质细胞中表达呈正相关 (r=0 .46,P <0 .0 1)。结论 SiO2
ObjectiveTo study the expression and locat ion of early growth response gene-1(Egr-1),transforming growth factor-β 1(T GF-β 1),fibronectin(FN) in silicotic rat and to discuss the role of Egr-1 in the development of silicosis. MethodsSilicotic animal m odel of rat was established,and the expressions of Egr-1,TGF-β 1,FN in vario us lung cells of silicotic rat were analysed by using immunohistochemical techni que(SP) and the image analysis. ResultsThe expressions o f Egr-1 in bronchial epithelial cell,pulmonary macrophage,alveolar epithelium c ell and interstitial cell in lung silicotic tissue(gray values:118.58±5.65～168 .52±5.67) were higher than those of controls(gray values:166.23±5.23～188.12± 8.35) during 1～28 days,and the expression was mainly in nucleus;the expressions of TGF-β 1 in these cells(gray values:123.49±5.65～170.24±3.56) were also higher than those of controls(166.53±6.25～198.56±4.53),and the expression was mainly in cytoplasm.The expressions of FN in bronchial epithelial cell,pulmonar y macrophage and alveolar epithelial cell(gray values:150.32±6.54～201.54±7.38 ) were lower,while those in interstitial cell(gray values:121.43±5.65～167.55± 6.35) were higher than those of controls.The changes of TGF-β 1 and Egr-1 ex pression level in bronchial epithelial cell,pulmonary macrophage,alveolar epithe lium cell and interstitial cell were synchronous during the experiment(1～28 day s).Both of them were correlated with each other( r =0.61, P <0.01),while t he expression of FN was not correlated with Egr-1,but correlated to TGF-β 1 in interstitial cell( r =0.46, P <0.01). Conclusion Silicon dioxide could up-regulate the expression of nuclear transcription facto r Egr-1 in several kinds of cell in lung.The activated Egr-1 may coordinate th e expression of TGF-β 1 and FN to regulate the development of silicosis.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2004年第1期47-50,i001,共5页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金资助项目 ( 30 1 70 399)
