摘要
目的探讨肺组织中基质金属蛋白酶(MMP-2/9)和其抑制物基质金属蛋白酶组织抑制因子(TIMP-1/2)在SiO2致矽肺纤维化中的表达及意义。方法清洁级Wistar大鼠64只,随机分为对照组和染尘组。采用暴露式气管内注入法染尘,染尘组每只大鼠注入1 ml SiO2混悬液,对照组注入1 ml灭菌生理盐水。染尘后3,7,14和28 d处死取其肺组织,光镜观察其病理切片;通过ELISA法,测定大鼠肺组织中MMP-2、MMP-9和TIMP1、TIMP-2蛋白的表达。结果与正常对照组比较,染尘组大鼠染毒后3 d肺组织发生局灶性病变,肺泡间隔增厚,间质细胞明显肿胀,局部可见肺泡腔形成细胞性结节,28 d时染尘组大鼠肺泡结构破坏或消失,肺组织以胶原沉积、肺纤维化改变为主,可见巨大纤维性结节;染尘组肺组织内MMP-2,MMP-9,TIMP-1和TIMP-2表达均较对照组增高,结果经秩和检验差异具有统计学意义(P<0.05)。结论矽肺组织内MMP-2/9和TIMP-1/2的高表达,说明其参与了矽肺纤维化的形成。
Objective To investigate the expression of matrix metalloproteinase（MMP-2/9） and tissue inhibitor of matrix metalloproteinase（TIMP-1/2） protein in silica-induced pulmonary fibrosis rats and its significance.Methods Sixty-four Wistar rats were randomized into control group and silica group.The rats were intratracheally injected with silica to establish the pulmonary fibrosis model in silica group,and the rats were given the same dose of saline by the same way in control group.After injection for 3,7,14 and 28 d,the rats were killed and lungs were collected.Histopathological changes of the lungs were observed.The protein expression of MMP-2,MMP-9 and TIMP-1,TIMP-2 was determined by enzyme-labeled immunosorbent assay.Results Compared with control group,the lung tissues presented with local lesions,broadened alveolar wall and septum,swelled interstitial cells,and local cellular nodules in pulmonary alveolar cavity in silica group at 3 d.At 28 d,the alveolar structures were damaged or disappeared in silica group and parts of the alveolar spaces were collapsed and replaced by collagens and fibroblasts.The protein expression of MMP-2,MMP-9,TIMP-1,and TIMP-2 in silica group was significantly higher than that in control group（P〈0.05）.Conclusion The results suggest that the MMP-2,MMP-9,TIMP-1,and TIMP-2 protein may be involved in the development of silica-induced lung injury fibrosis.
出处
《山西医科大学学报》
CAS
2011年第12期954-956,1020,共4页
Journal of Shanxi Medical University
基金
山西省实验动物专项基金资助项目(2007k03)
关键词
矽肺
肺纤维化
基质金属蛋白酶
基质金属蛋白酶组织抑制因子
silicosis
pulmonary fibrosis
matrix metalloproteinase
tissue inhibitor of matrix metalloproteinase
