摘要
目的观察五味子乙素(Sch-B)对染矽尘大鼠肺组织一氧化氮(NO)水平和诱导型一氧化氮合酶(iNOS)mRNA动态变化的影响。方法将96只大鼠随机分为对照组、染矽尘组、Sch-B组,每组32只,气管暴露法建立大鼠矽肺模型,造模后d 1开始灌胃给予Sch-B治疗,药物治疗3 d、7 d、14 d和28 d后,HE染色检测肺组织病理改变;硝酸还原酶法测肺组织NO含量;RT-PCR检测肺组织iNOS mRNA的表达。结果 HE染色显示Sch-B组大鼠肺损伤较染矽尘组明显减轻。NO含量和iNOS mRNA表达在染尘后各个时间点均较相应的对照组明显升高(P<0.01),其中NO含量在d 7时达到高峰后开始下降,iNOS mRNA的峰值出现在d 14。五味子组与染矽尘组相比,各时间点NO含量均明显降低(P<0.05或P<0.01),而iNOS mRNA表达仅在d 3和d 7时降低明显(P<0.05)。结论染矽尘大鼠肺组织存在着NO含量和iNOS mRNA的动态变化。Sch-B能减轻染矽尘大鼠肺组织的纤维化程度,其机制可能与染尘初期Sch-B降低iNOS mRNA转录水平,抑制NO炎症介质的合成与释放有关。
Aim To investigate the effect of schisandrin B on content of nitric oxide（NO）and expression of inducible nitric oxide synthase（iNOS）in rat lungs exposed to silica.Methods Ninty six Wistar rats were randomly divided into control group,silica group and Sch-B treated group with 32 rats in each group.Silicotic animal models were established by direct tracheal instillation of silica into rat lungs surgically.From the first day after model establishment,rats in Sch-B treated group were orally given Sch-B.At 3,7,14 and 28 days after treatment,HE staining was used to observe pathological changes of lung.The content of NO in lung homogenates was measured by the method of nitric acid reductase.The expression of iNOS mRNA was detected by RT-PCR.Results HE staining showed that the pulmonary injury in rats was significantly relieved in the Sch-B group compared with silica group.The content of NO and expressions of iNOS mRNA in lung homogenates of silica group were higher than those of control group（P0.01）.The content of NO reached its summit at d 7,while the expressions of iNOS mRNA reached its summit at d 14.The content of NO in Sch-B group was significantly lower than that of silica group,while the expressions of iNOS mRNA in Sch-B group were markedly decreased compared with those silica group at d 3 and d 7.Conclusions Dynamic change of content of NO and mRNA expression of iNOS can be seen in rat lungs exposed to silica.Sch-B can ameliorate the silica induced pulmonary fibrosis,and its mechanism is probably related to its effect of reducing the expressions of iNOS mRNA and inhibiting content of NO at the initial stage of rat lungs exposed to silica.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2011年第2期225-228,共4页
Chinese Pharmacological Bulletin
基金
山西省实验动物专项基金项目(No2007k03)
