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瘦素预处理减轻小鼠心肌缺血／再灌注损伤的实验探讨 被引量：11

Experimental study on myocardial ischemia reperfusion injury reduction in mouse by leptin pretreatment
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摘要 目的监测小鼠心肌缺血／再灌注损伤（MIRI）过程中，血清炎性细胞因子TNF—α、IL-6水平，中性粒细胞活性及氧自由基含量的变化，以及瘦素（1eptin）预处理对其影响，探讨瘦素预处理在小鼠MIRI过程中的作用及其机制。方法24只雄性C57BL／6小鼠随机分为四组：①假手术组；②心肌缺血／再灌注模型组（MIRI组）；③MIRI瘦素预处理组（leptin＋MIRI组）；④MIRI术后瘦素处理组（MIRI＋leptin组）。在MIRI手术后6h，四组小鼠分别取标本，测定小鼠血清炎性细胞因子水平（TNF-α、IL-6）、髓过氧化物酶活性（MPO）水平、血清丙二醛（MDA）活性以及超氧化物歧化酶（SOD）含量。结果瘦素预处理可降低细胞因子TNF—α、IL-6的水平。降低小鼠MPO活性，减少MDA含量并升高SOD活性。结论瘦素预处理可减少中性粒细胞的聚集与浸润，减轻脂质过氧化和自由基损伤，对MIRI有保护作用。 Objective To investigate the mechanism of the effect of leptin pretreatment on myocardial isehemia reperfusion injury （MIRI）in mouse. Methods Twenty four male C_.57BL/6 mice were randomized into 4 group： ①the shamoperation group; ②the myocardial ischemia reperfusion group （ MIRI group ）, established by blocking left coronary artery anterior descending branch for 30 rain followed by reperfusion;③the leptin pretreatment group （Leptin ＋ MIRI group）, established by injecting intraperitoneally leptin at 30 min before ischemia; ④the leptin post - treatment group （ MIRI ＋ Leptin group ） ; established by injecting intraperitoneally leptin at 30 min after reperfusion. Blood and heart samples were harvested from each mouse at 6 hours after reperfusion. We then analyzed the samples for tumor necrosis factor - alpha （ TNF - α） and interleukin - 6 （ IL - 6 ） levels associated with MIRI, myeloperoxidase （ MPO ） level, malondialdehyde （MDA） contents and superoxide dismutase （ SOD ） activity. Results Administration of leptin before MIRI significantly reduced serum TNF - α and IL - 6 levels, decreased myeloperoxidase （MPO） level, improved malondialdehyde （MDA） content and restored superoxide dismutase （ SOD） activity. Conclusion Pretreatment of leptin showed protective effects on mouse MIRI by Ameliorating the up - regulation of leptin level in mouse MIRI model. Up - regulation of leptin level induced by MIRI deteriorates myocardial injury.
作者 徐彤彤 李芳
机构地区 桂林医学院附属医院特需病区
出处 《中国急救医学》 CAS CSCD 北大核心 2009年第8期727-730,共4页 Chinese Journal of Critical Care Medicine
基金 2008年桂林市科学研究与技术开发项目（N0.科技攻关20080211） 2009年广西科学研究与技术开发计划课题（No.桂科攻0993003C-18） 2008年广西教育厅项目（No.200810LX464）
关键词 心肌缺血/再灌注 瘦素 Myocardial ischemia repeffusion Leptin
分类号 R542.2 [医药卫生—心血管疾病]
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参考文献14

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二级参考文献30

  • 1孙志军,余勤,马淑梅,杨志勇,孙英贤.急性心肌梗死患者血清瘦素、肿瘤坏死因子-α浓度的变化[J].中国新药杂志,2004,13(8):744-746. 被引量：1
  • 2林季,颜光涛,郝秀华,王录焕,张凯,薛辉.Leptin及Orexin-ARIA在大鼠肠缺血-再灌注损伤中的应用[J].放射免疫学杂志,2004,17(5):329-332. 被引量：7
  • 3Fujimaki S,Kanda T,Fujita K,et al.The significance of measuring plasma leptin in acute myocardial infarction[J].J Int Nkd Res,2001;29:108-113.
  • 4Chu NF,Spiegelman D,Botamisligil GS,et al.Plasma insulin,leptin,and soluble TNF receptors levels in relation to obesity-related atherogenic and thrombogenic-cardiovascular disease risk factors among men[J].Atherosclerosis,2001;157:495-503.
  • 5Christopher J,Ronald E,Willa AH.Adiposity,inflammation atherogenesis[J].Endocrinology,2003;144:2195-2200.
  • 6Bouloumie A,Nbrumo T,Lafontan M.Leptin induces oxidative stress in human endothelial cells[J].FASEB J,1999;13:1231-1238.
  • 7Yamagishi SI,Eselstein D,Du XL,et al.Leptin induces mitochondrial superoxide production and monocyte chemoattractant protein-1 expression in aortic endothelial cells increasing fatty acid oxidation via protein kinase[J].J Biol Chem,2001;276:25096-25100.
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  • 10Barouch LA,Berkowitz DE,Harrison RW,et al.Disruption of leptin signaling contributes to cardiac hypertrophy independently of body weight in mice[J].Circulation,2003;108:754-759.

共引文献11

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二级引证文献78

中国急救医学
2009年 第8期

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