摘要
目的:研究瘦素(leptin)在肝缺血/再灌注(H-I/R)后肠道内的表达变化,并探讨leptin在H-I/R介导的肠道损伤中的作用.方法:建立大鼠70%H-I/R模型,大鼠随机分成5组,每组9只,依次为假手术(Sham)组、肝缺血60min/再灌注60min(I60'R60')组、I60'R150'组、I60'R240'组和I60'R360'组.采用酶比色法检测损伤后血清二胺氧化酶活性,采用HE染色和免疫组织化学法分别检测损伤后十二指肠的病理改变和leptin蛋白表达,并采用逆转录-PCR法观察损伤后十二指肠leptinmRNA表达的变化.结果:与损伤后Sham组比较,四个再灌注组血清二胺氧化酶活性无显著差异,但I60'R60'组显著高于I60'R360'组(P=0.0077).病理学观察提示H-I/R早期的十二指肠损伤较重而后期逐步减轻.与损伤后Sham组十二指肠leptin蛋白表达相比,I60'R240'、I60'R360'组显著升高(0.126503±0.005873,0.129458±0.003755vs0.079269±0.001995,均P<0.01),再灌注组之间按I60'R360'、I60'R240'、I60'R60'、I60'R150'组的顺序依次递减.与损伤后Sham组十二指肠leptinmRNA表达相比,I60'R150'组显著降低(0.944±0.033vs1.022±0.011,P=0.049).结论:Leptin在H-I/R后肠道内发生的表达变化与肠道损伤密切相关,提示leptin可能作为一种保护因子对抗H-I/R介导的肠道损伤.
AIM： To explore the changes of leptin in intestinal tract following hepatic ischemia/ reperfusion （H-I/R）, to investigate the association between these changes and H-I/ R-induced intestinal injury, and to find out the role of leptin in H-I/R-induced intestinal injury.
METHODS： A 70% H-I/R model of rats was established, forming 5 groups including shamoperation and injury ones based on different reperfusion time. Enzyme-colorimetry was used to detect serum diamine oxidase activity after injury, hematoxylin-eosin staining and immunohistochemistry were applied to investigate pathological variations and leptin protein expressions in duodenum after injury, respectively, while reverse transcriptase-PCR was used to detect leptin mRNA expressions in duodenum after injury.
RESULTS： Compared with sham-operation group after injury, the four reperfusion groups showed no significant difference in serum diamine oxidase activity, but serum diamine oxidase level was significantly higher in 60 min ischemia/60 rain reperfusion （I60′R60′） group than in I60′R360′ group （P = 0.0077）. Patho- logical investigation suggested that duodenal impairments at the early phase of H-I/R were more serious, while the impairments at the later phase lessened gradually. Compared with leptin protein expression in duodenum of sham-operation group after injury, that of I60′R240′ and I60′ R360′ groups increased significantly （0.126503 ± 0.005873, 0.129458 ± 0.003755 vs 0.079269 ± 0.001995, both P 〈 0.01）, and the levels of reperfusion groups decreased in such order as I60′ R360′, I60′R240′, I60′R60′ and I60′R150′ groups. Compared with leptin mRNA expression in duodenum of sham-operation group after injury, that of 160′R150′ group decreased significantly （0.944±0.033 vs 1.022± 0.011, P = 0.049）, and it was significantly lower than the level of I60′ R360′ group.
CONCLUSION： The expression changes of leptin in intestinal tract after H-I/R are closely associated with intestinal injury, suggesting that leptin may be a protective factor of resisting H-I/R-induced intestinal injury.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第31期3485-3491,共7页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30670821
中国人民解放军总医院苗圃基金资助项目
No.06MP83~~
