摘要
目的 :研究肠缺血再灌注损伤对血清及脂肪组织 L eptin水平的影响 ,探讨 L eptin在急性炎症反应中的作用。方法 :建立大鼠肠缺血再灌注损伤模型 ,采用高灵敏鼠类 L eptin放射免疫分析方法测量血清及脂肪组织 L eptin浓度变化。结果 :肠缺血 6 0 min、再灌注损伤 30 m in后血清 L eptin水平为 (1 0 .82± 0 .83) μg/L,相对于实验前的 (1 6 .4 6± 3.2 1 ) μg/L 有显著下降 (P<0 .0 5 ) ,而血清 L eptin浓度高于脂肪组织水平 (4 .4 6±2 .6 3) m g/1 0 0 g,并且两者的 L eptin水平都有随损伤时间延长而逐步升高的趋势 (P=0 .0 4 7)。结论 :L eptin可能具有炎性细胞因子的作用 。
Objective: To explore the effect of intestinal ischemia/reperfusion injury on Leptin concentrations in serum and adipose tissue, and to find out the role of Leptin in acute inflammatory responses. Methods : An intestinal ischemiareperfusion injury model of rats was established, and used a highly sensitive murine Leptin radioimmunoassay to check the change of Leptin concentrations in serum and adipose tissue. Results : Serum Leptin level(10 82±0 83)μg/L significantly decreased after an injury of 60minute ischemia and 30minute reperfusion versus preexperimental serum levels (16 46±3 21)μg/L; Leptin level in serum was higher than that in adipose tissue (4 46±2 63)mg/100 g, and they both showed a similar changeable trend to increase step by step as reperfusion time extended( P =0 047). Conclusion: Leptin may be an inflammatory cytokine and may play a role in inflammatory responses such as intestinal ischemia/ reperfusion .
出处
《中国危重病急救医学》
CAS
CSCD
2003年第12期722-725,共4页
Chinese Critical Care Medicine
基金
国家自然科学基金资助项目 ( 3 9970 717)
