摘要
从大连潮间带繁茂膜海绵中分离到一株具有很强抑制革兰氏阳性菌活性和较强抗肿瘤活性的链霉菌。通过其形态观察 ,生理生化测定和 1 6SrDNA序列的测定 ,初步判定属于假浅灰链霉菌 (Streptomycespseudogriseolus)。并对其活性物质的生产条件进行了初步探索和优化 ,确定了用天然海水配制的TSB培养基为最佳发酵培养基 ,3d为最适发酵时间。
An actinomycete B37 was isolated from an intertidal marine sponge Hymeniacidon perleve, which has strong activity against Gram positive bacteria and moderate activity against tumor cells. The mycelium and spore morphology, physiological properties and 16SrDNA sequence suggested that B37 is Streptomyces pseudogriseolus. The fermentation conditions of this strain were investigated for the biosynthesis of bioactive metabolites.
出处
《微生物学通报》
CAS
CSCD
北大核心
2004年第5期60-64,共5页
Microbiology China
基金
国家"973"计划资助项目 (No 2 0 0 3CB71 60 0 1 )
关键词
繁茂膜海绵
假浅灰链霉菌
放线菌
活性
鉴定
Marine sponge Hymeniacidon perleve,Streptomyces pseudogriseolus,Actinomyces,Activity,Identification
