摘要
采用平板涂布法从我国南海三亚周边海域贪婪倔海绵(Dysidea avara)中分离海绵共附生细菌,采用金黄色葡萄球菌、大肠埃希氏菌、荧光假单胞菌、枯草芽孢杆菌、白假丝酵母、宛氏拟青霉、黑曲霉7种指标菌进行抑菌试验筛选抗菌活性菌,同时对于得到的活性菌进行生理生化鉴定。共分离获得个149个细菌菌株,发现20株具有抑制真菌和革兰氏阳性细菌的活性,占细菌总数的13.4%。经过细菌形态观察和生理生化试验,发现此20株活性菌属于革兰氏阳性芽孢杆菌属(Bacillussp.)。
Sponge Dysidea avara-associated bacteria were isolated by dilution-plate method and the bacterial anti- microbial activities were screened for Escherichia coli, Staphylococcus aureu, Pseudomonas fluorescens, Bacillus subtilis, Aspergillus niger, Candida albicans, Pacecilomyces variotii by agar diffusion method. At the same time, morphology observations, biochemical and physiological identification of active strains were made. In this paper, 149 strains were isolated from sponge Dysidea avara. 20 strains, which amount to 13.4% of the total isolated strains, were found to have anti-microbial activity against fungi and gram-positive bacteria and were identified to be the genus Bacillus sp.
出处
《微生物学通报》
CAS
CSCD
北大核心
2005年第4期15-19,共5页
Microbiology China
基金
国家高技术研究发展计划项目("863"项目)(No.2004AA628060)
上海市科学技术委员会青年科技启明星计划资助(No.04QMX1411)
上海市高校优秀青年教师后备人选计划资助
关键词
贪婪倔海绵
细菌
抗菌活性
分类学
Dysidea avara, Bacteria, Anti-microbial activity, Taxonomy
