摘要
目的 研究地黄饮子对大鼠持续性脑缺血后海马内脑源性神经营养因子 (BDNF)的影响。方法 用线栓法建立大鼠脑缺血模型 ,以免疫组化技术显示地黄饮子在缺血后对海马区BDNF的影响。结果 在大鼠脑缺血区有BDNF阳性神经元表达 ,缺血组和给药组海马区神经元随缺血时间的延长 ,存活细胞数目均逐渐减少 ,而给药组存活的神经细胞数目较缺血组多。结论 地黄饮子可通过促进BDNF表达 ,抑制细胞死亡 ,促进脑缺血后海马神经元的存活和修复。
Objective To study the effects of Dihuangyinzi Decoction (DD) on brain-derived neurotrophic factor (BDNF) in the hippocampus of the rat with persistent ischemia. Method The rat model of ischemia was established by ligation. The effects of DD on BDNF in the hippocampus of the rat model of ischemia were observed by using immunohistochemical techniques.Results It was found that there was the expression of BDNF-positive neurons in the ischemic region of the rat brain;and although the survived neurons gradually reduced along with the prolongation of the ischemia time in the hippocampal regions of the rats in both the ischemia group and DD-administration group,the survived neurons in the DD-administration group were more than those in the ischemia group. Conclusion DD can inhibit the death of the neurons and promote the survival and repair of the neurons in the hippocampus through promoting the expression of BDNF in the cerebrally ischemic rat
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2004年第4期64-67,F003,共5页
Journal of Beijing University of Traditional Chinese Medicine
关键词
脑源性神经营养因子
地黄饮子
脑缺血
免疫组化
大鼠
Brain Derived Neurotrophic Factor
Dihuangyinzi Decoction
Cerebral Ischemia
Immunohistochemistry
Rat
