摘要
目的 作者用物理化学方法自制新型的陶瓷化骨材料 ,测试其理化性质及力学强度 ,为骨组织工程研究提供可供选择的支架材料。方法 将猪肋骨分为未处理骨、脱蛋白骨和脱蛋白脱脂骨三组 ,经不同煅烧温度及时间处理得到多种陶瓷化骨。用扫描电镜观察其形貌特征 ,用X -射线能谱和X -射线衍射分析材料成份 ,并对材料的力学性能进行分析测定。结果 各种处理所得到的陶瓷化骨保持原骨组织的天然网状孔隙系统 ;所含的原子均只有钙和磷 ,比值约 1 72 ;主要成份均为羟基磷灰石 ;90 0℃煅烧 2h以上条件所得陶瓷化骨中的羟基磷灰石呈细结晶状态 ,有较高抗压强度 ,90 0℃煅烧 1h以下条件所得骨抗压强度很低。结论 猪骨通过不同预处理后煅烧所得的陶瓷化骨理化性能相同 ,皆保持天然多孔结构 ,90 0℃煅烧 2h以上有较好抗压性能 ,仅含钙磷原子 ,化学成份为细结晶羟基磷灰石 ,但是否是一理想支架材料 ,尚需要进一步验证其降解性和组织相容性。
Objective To detect the properties of new natural porcine ture bones ceramic(TBC) which were processed under different conditions. Methods Fresh porcine ribs were pretreated and divided into three groups: untreated group, deproteinization group and deproteinization and defat group. Then they were calcined under different temperature and duration to get TBC. The morphological features, constitutive components and mechanical properties were examined by scanning electron microscopy, X-rays diffraction analysis, energy dispersive X-ray microanalysis (EDX), mechanical assay and so on. Results All TBC possessed the natural porous network system of original bones. EDX showed that the all elements of TBC were Ca and P, the ratio of which was 1.72, and the major mineral composition of TBC was hydroxyapatite(HA). The TBC calcined at the higher than 900℃ temperature for 2 hours had comparative good mechanical strength, otherwise the mechanical strength was too low to act as scaffold materials. Conclusion Morphological features, constitutive components and mechanical properties of differently pretreated TBC were same. The TBC calcined at the temperature of higher than 900℃ for 2 hours had comparative good mechanical strength, and contained only HA with high crystallization. Befroe the TBC becoming an ideal scaffold material, more investigations will be necessary to detect its biocompatibility and biodegradability.
出处
《中国医师杂志》
CAS
2004年第8期1014-1016,共3页
Journal of Chinese Physician
基金
国家自然基金 (30 2 0 0 0 64)
湖南省自然科学基金 (0 3JJY30 2 7
0 2JJY2 0 51 )
