摘要
采用溶胶凝胶法在58S生物玻璃的基础上用氧化锌取代3 mol%的氧化钙制备了含锌的生物玻璃粉体(58S3Z),对合成的粉体采用有机泡沫浸渍法在700℃及1200℃制备出58S3Z-700℃、58S3Z-1200℃玻璃及玻璃陶瓷多孔支架。在所得支架表面涂覆PLGA及PBS薄膜制备出58S3Z-1200℃-PLGA及58S3Z-1200℃-PBS复合支架。对其形貌、孔隙率、力学性能、体外降解性及细胞相容性进行了系统研究。复合后多孔支架仍然保持三维连通的多孔结构,孔隙率与复合前(86.9%±0.8%(58S3Z-700℃),80.1%±0.6%(58S3Z-1200℃))相比稍有下降,分别为75.9%±0.6%(58S3Z-1200℃-PLGA)和77.9%±0.9%(58S3Z-1200℃-PBS)。但复合多孔支架显示出较高的抗压强度,分别达到1509.4 kPa±162.8 kPa(PLGA)和901.6 kPa±94.5 kPa(PBS),与玻璃和玻璃陶瓷支架(258.4 kPa±23.6 kPa)相比具有较大的提高。体外降解实验表明58S3Z-1200℃-PLGA、58S3Z-1200℃-PBS复合多孔支架可降解,经过28天的浸泡其失重率分别达到13.3%和2.1%。体外研究结果表明:58S3Z玻璃陶瓷支架复合PBS或PLGA后支持成骨细胞黏附、铺展和生长。这种新型的复合支架具有三维的网状多孔结构,良好的力学性能、降解性和细胞相容性,有望成为一种较理想的骨组织工程支架。
The porous Zn-containing glass and glass- ceramic scaffolds were prepared by sintering a ceramic slipcoated polymer foam at 700℃ and 1200℃, and the PLGA （poly-lactic-glyoclic-acid） or PBS （poly （butylene succinate）） glass - ceramic composite scaffolds were obtained by coating a PLGA and PBS film on the framework of the glass - ceramic scaffolds. The obtained glass -ceramic scaffolds have a well-interconnected porous structure and show high porosity of 80.1% ± 0.6 % and low compressive strength of 258.4 kPa± 23.6 kPa. The coating of PLGA or PBS film on glass - ceramic scaffolds does not affect interconnection of the pores, but results in a decrease of porosity and a sharp increase of compressive strength. The porosity and compressive strength of the scaffolds were 75.9%±0. 6% and 1509.4 kPa±162.8 kPa for 58S3Z- 1200℃-PLGA, and 77.9%±0. 9%, 901. 6 kPa±94.5 kPa for 58S3Z - 1200℃- PBS. The in vitro degradation experiments show that the composite scaffolds are degradable, reaching up to 13.3% （58S3Z - 1200℃- PLGA） and 2.1% （58S3Z - 1200℃- PBS） after 28 days of soaking. The cellular responses were assessed by culturing mesenchymal stem cells （MSC） on the scaffolds, and SEM observations show that the cells are attached to and proliferate well on the scaffolds. The results suggest that the composite scaffold has porous structure, higher mechanical strength, good degradability and cell compatibility,and might be used for bone tissue engineering.
出处
《复合材料学报》
EI
CAS
CSCD
北大核心
2008年第6期123-129,共7页
Acta Materiae Compositae Sinica
基金
国家973组织工程重大专项项目(2005CB522700)
上海市基础研究重点项目(02JC14009)
关键词
生物玻璃
聚酯
多孔支架
降解
细胞相容性
bioactive glass
polyester
porous scaffold
degradation
cell biocompatibility
