摘要
本论文研究硒酸多糖对二乙基亚硝胺(diethylnitrosamine,DEN)致小鼠肝癌的预防作用。采用50μL/kgDEN诱发小鼠肝癌,每周皮下注射1次,连续8周,同时对照组饮用灭菌水,低硒含量组和高硒含量组分别饮用含不同硒量的硒酸多糖(150mg/kg)。实验结束后,处死小鼠,测定生化指标等,用比色分析法检测肝匀浆SOD、GSH-Px的活性及MDA含量的变化。病理学检查发现,对照组肝脏损坏严重,补硒组则较轻;2个补硒组能显著提高SOD、GSH-Px的活性,降低MDA含量。硒酸多糖对DEN致小鼠肝脏癌变有抑制作用。
The inhibitory effect of selenium-polysaccharide on diethylnitrosamine （DEN） induced hepatocarcinoma in mice was investigated. Mice hepatocarcinoma was induced by subcutaneously injecting 50μL/kg diethykinitrosamine （DEN） once a week for 8 weeks. Mice were fed with distilled water for control group and different dosages of selenium-polysaccharide for low selenium supplementation group and high selenium supplementation group. Then the biochemical indexes of the killed mice were measured. The activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and the concentrations of malondialdehyde （MDA） were investigated by colorirnelric method. It was found that selenium-polysaccharide significantly increased the activities of SOD, GSH-Px and decreased MDA content in liver cell homogenate. Pathology analysis also showed that damage of mice liver in control group was more serious than those in the selenium supplementation groups. Selenium-polysaecharide had inhibitory effect on DEN-induced hepatocarcinoma in mice.
出处
《现代食品科技》
EI
CAS
2008年第8期748-750,共3页
Modern Food Science and Technology
基金
国家自然科学基金资助项目(20776113)
关键词
硒酸多糖
二乙基亚硝胺
肝肿瘤
selenium-polysaccharide
diethylnitrosamine
hepatocarcinoma
