摘要
目的 :研究糖皮质激素对小鼠支气管哮喘 (简称哮喘 )模型肺组织粘蛋白基因Muc5acmRNA和蛋白表达的影响 ,探讨其对哮喘时气道粘液过度分泌的作用。方法 :1 8只清洁级BALB/c小鼠被随机分为对照组、哮喘组和地塞米松组 ,每组 6只。后 2组制作哮喘模型 ,地塞米松组用地塞米松治疗。采用RT PCR和免疫组化法分别检测肺组织中Muc5acmRNA和Muc5ac蛋白的表达。结果 :哮喘组肺组织Muc5acmRNA(0 .5 341± 0 .0 30 3)和蛋白 (0 .1 90 6± 0 .0 0 0 8)表达均较对照组 (分别为 0 .1 994± 0 .0 1 2 8和 0 .1 1 94± 0 .0 0 0 7)明显增加 (P均 <0 .0 1 ) ,地塞米松治疗后 2者均明显降低 (分别为 0 .2 72 9± 0 .0 345和 0 .1 4 5 6± 0 .0 0 0 3)。结论 :地塞米松可下调Muc5acmRNA和蛋白表达 。
Aim: To study the effects of glucocorticoid on the expression of Muc5ac mRNA and protein in asthmatic mice lung, and to investigate its role in mucus secretion of airways. Methods: Eighteen pathogen free BALB/c mice were randomly divided into control group, asthmatic group, and dexamethasone group. Dexamethasone group was treated with dexamethasone.The expression of Muc5ac mRNA and Muc5ac apomucin in lung were detected by the methods of reverse transcription polymerase chain reaction (RT PCR) and immunohistochemical assay, respectively. Results: The expression of Muc5ac mRNA(0.534 1±0.030 3)and protein (0.190 6±0.000 8) in asthmatic group were significantly higher than those of control group(0.199 4±0.012 8 and 0.119 4±0.000 7,respectively)and reduced(0.272 9±0.034 5 and 0.145 6 ±0.000 3,respectively)after treatment with dexamethasone. Conclusion: Dexamethasone substantially downregulates Muc5ac mRNA and protein expression, which may have potential therapeutic role in mucus overproduction of airways in asthma.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2004年第4期610-612,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
博士研究生课题
