摘要
目的:观察哮喘小鼠肺和下丘脑组织中GABAARα表达的变化。方法:成年清洁级雌性BALB/C小鼠30只,按随机数字表法分为对照组、哮喘组(卵蛋白致敏)和地塞米松组(卵蛋白致敏,激发前腹腔注射地塞米松1mg/kg进行干预),每组10只。对小鼠肺组织进行病理学观察和糖原染色,用免疫组化SP法、RT-RCR检测肺组织及下丘脑中GABAARα蛋白和mRNA的表达水平,RT-RCR法测肺组织中MUC5AC mRNA的表达。结果:哮喘组小鼠气道黏膜充血水肿,黏膜层增厚,支气管壁及血管壁周围有较多炎症细胞浸润,杯状细胞增多;地塞米松组上述表现较哮喘组减轻。3组小鼠肺组织中GABAARα mRNA和蛋白的表达、下丘脑组织中GABAARα mRNA和蛋白的表达、肺组织中MUC5ACmRNA的表达差异均有统计学意义(F=4.762、113.196、145.468、54.860和128.947,P<0.01);哮喘组上述指标均高于对照组(P<0.05);地塞米松组上述指标均低于哮喘组(P<0.05),但仍高于对照组(P<0.05)。哮喘组小鼠下丘脑和肺组织中GABAARα蛋白表达水平、mRNA的相对表达量呈正相关(r分别为0.791、0.740,P<0.05),肺组织中MUC5AC与GABAARα mRNA的相对表达量呈正相关(r=0.814,P<0.01)。结论:GABAARα高表达可能诱发气道黏液的过度分泌,与哮喘发病密切相关;GABA系统可能与哮喘的中枢神经调节有关。
Aim：To explore the changes of GABAARα expression in asthmatic mice lung and hypothalamus tissues.Methods：A total of 30 mice were randomly divided into control group,asthmatic group and dexamethasone group,and 10 mice in each group.GABAARα mRNA and protein in lung and hypothalamus tissues were analyzed by RT-RCR and immunohistochemistry,the variation of MUC5AC mRNA in the lung tissue was detected by RT-RCR.Results：Mice in asthma group showed a significant hyperplasia of goblet cell and severe airway inflammation.These changes could be inhibited in dexamethasone group.The levels of GABAARα mRNA and protein in lung tissue and hypothalamus tissue,MUC5AC mRNA in lung tissues differed from each other among the 3 groups（F=4.762,113.196,145.468,54.860,128.947,P0.01）.The indexes mentioned above in asthma and dexamethasone groups were higher than those of the control（P0.05）,and those in dexamethasone group were lower than asthma group（P0.05）.In asthma group,the expression of GABAARα in lung tissues was positively correlated to those in hypothalamus（correlation coefficient r of protein and mRNA were 0.791,0.740,respectively,P0.05）,the GABAARα mRNA expression was positively correlated to MUC5AC mRNA in lung tissue（r=0.814,P0.01）.Conclusion：GABA system is related to asthma.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2012年第2期218-223,共6页
Journal of Zhengzhou University(Medical Sciences)
