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用一个SSR标记快速检测小麦杂种后代Waxy基因型 被引量:13

Rapid Classification of Partial Waxy and Waxy Wheats Using a SSR Marker
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摘要 为了提高Wx蛋白的检测手段,通过对几个已知Waxy基因型的品种(系)的分子标记检测和蛋白电泳谱带分析,发现一个特异的SSR标记能同时对三个Wx基因位点进行检测;同时用这个特异的SSR标记对一个糯小麦Et-05及普通栽培小麦扬麦158的杂交后代F2进行分析,发现了4株糯小麦植株。 Several PCR-based methods for the analysis of GBSS alleles have been developed for application in wheat. This paper describes a SSR primer for all three Wx loci that can be used under a single reaction, (allowing) 96 lines to be characterized in a single PCR run using a 96-well plate. The PCR selection method described here is an easy and effective way for identification of null alleles.
出处 《麦类作物学报》 CAS CSCD 2004年第3期9-12,共4页 Journal of Triticeae Crops
基金 国际合作项目(BE2003049) 国家863项目(2001AA211021) 江苏省高新技术研究项目(BG2002304)。
关键词 糯小麦 SSR标记 标记辅助育种 Waxy wheat SSR marker Marker-assisted selection
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参考文献25

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二级参考文献8

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