摘要
为探讨纤维蛋白原Bβ链基因启动子区多态性位点Bβ - 4 5 5G A与高血压及其缺血性并发症的关系 ,应用聚合酶链反应—限制片长多态性技术对 14 9例高血压患者、12 4例高血压合并冠心病患者和 14 8例年龄、性别相匹配的健康人群进行纤维蛋白原 β - 4 5 5G A基因多态性分析 ;比浊法测定血浆纤维蛋白原水平。结果发现 ,冠心病组血浆纤维蛋白原水平显著高于高血压组 (P <0 .0 5 )和正常对照组 (P <0 .0 1) ,而高血压组与正常对照组之间差异无显著性 (P >0 .0 5 )。β - 4 5 5G A基因多态性分布在各研究组均符合Hardy Weinberg平衡定律。A等位基因频率在冠心病组 (0 .2 38)显著高于高血压组 (0 .171)和正常对照组 (0 .15 2 ;P均 <0 .0 5 )。各组内A等位基因携带者血浆纤维蛋白原水平显著高于GG基因型者 ,且冠心病组A等位基因携带者血浆纤维蛋白原水平更易受年龄等环境因素影响。Logistic回归分析发现 ,存在此突变位点的高血压患者发生冠心病的危险提高了 6 3.7%。结果提示 ,血浆纤维蛋白原水平增高是发生心血管疾病的重要危险因素 ,受遗传因素和环境因素共同影响。纤维蛋白原 β -4 5 5G A基因多态性可能通过改变血浆纤维蛋白原浓度影响冠心病的发生 ,说明遗传因素与冠心病发病相关。
Aim To explore the association of fibrinogen gene polymorphism with hypertension ischemic complications. Methods The Fibrinogen β-455G/A gene polymorphism was identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 149 hypertensive cases without complication, 124 hypertension cases with coronary heart disease and 148 matched controls by age and sex. Turbidimetric assays were performed to measure the plasma fibrinogen levels of all cases. Results The plasma fibrinogen level in coronary heart disease group was significantly higher than that in the controls (P<0.01) and hypertension patients (P<0.05). The A-allele was associated with elevated plasma fibrinogen levels in both patients and controls. In A-allele carriers with coronary heart disease, the older people had significantly higher plasma fibrinogen levels than the younger (P<0.05). The distribution of β-455G/A gene polymorphism was in accordance with the Hardy-Weinberg equilibrium (P>0.05). The A-allelic frequency in the coronary heart disease group was 0.238, significantly higher than that in the control group (0.152) and hypertension group (0.171; P<0.01), but there was no significantly difference between the control group and hypertension group (P>0.05). Logistic regression analysis showed that the relative risk to coronary heart disease in the hypertensions carrying A-allele (GA+AA genotype) increases 1.637 times. Conclusions The study demonstrates that fibrinogen is the important risk factor for the cardiovascular diseases. The plasma fibrinogen levels are affected by both genetic and environmental factors. The fibrinogen β-55G/A gene polymorphism may influence the progress of the coronary heart disease by regulating plasma fibrinogen levels. Genetic factor is associated with coronary heart disease.
出处
《中国动脉硬化杂志》
CAS
CSCD
2004年第2期199-202,共4页
Chinese Journal of Arteriosclerosis
