摘要
为了研究载脂蛋白Ｅ基因多态性及血脂与老年心肌梗死的关系，本文采用聚合酶链反应－限制片长多态性对９７例对照者和４９例心肌梗死患者载脂蛋白Ｅ基因型进行分析，同时检测血脂指标。结果发现，两组的Ｅ３／３基因型所占百分比最大，其次是Ｅ３／４基因型；对照组和心肌梗死组的ε２、ε３、ε４等位基因频率分别为０．０３０９、０．８９１８、０．０７７３和０．０３０６、０．８１６３、０．１５３１。心肌梗死组ε４携带者百分比大于对照组，Ｅ３／４基因型所占百分比大于对照组（Ｐ均＜０．０５）。与Ｅ３／３基因型相比，Ｅ３／４基因型在心肌梗死发生中的ＯＲ值为５．５２，９５％可信限为１．０２～２９．８０（Ｐ＜０．０５）。结果提示，Ｅ３／４基因型为心肌梗死发生的独立危险因子，女性心肌梗死的发生受基因型影响较男性更为明显。
Aim To study the relationship between apolipoprotein E (apo E) genotic polymorphisms,serum lipid and the incidence of myocardial infarction in the old people. Methods Apo E genotypes of 97 controllers and 47 myocardial infarction patients were genotyped by polymerase chain reaction restriction fragment length polymorphism and their serum lipid were measured at the same time. Results The most frequency genotype was E3/3 and the next was E3/4 in the two groups. The estimated apo E allele frequencies in the sample of control group were 0.0309, 0.8918 and 0.0773 for the ε2, ε3 and ε4 alleles, respectively. The estimated frequencies of the ε2, ε3 and ε4 alleles in the sample of myocardial infarction group were 0.0306, 0.8163 and 0.1531, respectively. The frequency of ε4 allele carriers in the myocardial infarction group was bigger than in the control group. The frequency of E3/4 genotype of myocardial infarction group was also higher than the control group (P<0.05). Compared to apo E genotype E3/3, a risk associated with genotype E3/4 increased in the myocardial infarction group. The OR of genotype was 5.52 with 95% CI 1.02～29.80(P<0.05) after adjusted by sex, age, and lipid pool. Conclusion Apo E genotype E3/4 was the independent risk factor for myocardial infarction in Shanghai. The genetic effect on the occurrence of myocardial infarction was more directly in women than in men.
出处
《中国动脉硬化杂志》
CAS
CSCD
1999年第2期110-113,共4页
Chinese Journal of Arteriosclerosis
基金
上海市自然科学基金
关键词
载脂蛋白E
基因多态性
血脂
PCR
心肌梗死
Apolipoprotein E
Genotypes
Gene Frequency
Polymerase Chain Reaction
Polymorphism,Restriction Fragment Length
Alleles
Myocardial Infarction
Patients
