摘要
化学发光免疫分析方法作为一项具有高特异性和高灵敏度的免疫分析方法,经过近几十年的发展,目前已在食品安全检测领域得到了良好的应用。本文根据不同化学发光免疫分析方法所选择标记物的不同,将化学发光免疫分析方法分为化学发光免疫分析、化学发光酶免疫分析和电化学发光免疫分析三个类别,并对各方法常用的化学发光标记物及分析方法原理进行介绍。同时,综述了化学发光免疫分析方法在农兽药残留、生物毒素、违禁添加物及微生物检测方面的应用进展情况。随着化学发光免疫分析方法在新型化学发光标记物及化学发光增敏体系方面取得进一步的研究进展,其在食品安全检测领域必将得到更为广泛的应用。
Chemiluminescent immunoassay (CLIA) as the technology with high specificity and sensitivity, after decades of development, has got a good application in the field of food safety detection. According to the different labelled substances used in the CLIA system, the methods were divided into CLIA, chemiluminescent enzyme immunoassay (CLEIA) and electrochemiluminescent immunoassay (ECLIA). This review briefly de-scribed the classical chemiluminescent labelled substances and the principles of various CLIA systems. Mean-while, the application of CLIA in the detection of pesticide residue, veterinary drug residue, biological toxins, prohibited addictives, and microorganisms were introduced. Accompanied with the further development of CLIA applied in novel chemiluminescent labelled substances and sensitized system, the technology will have a prosperous future in detection of food safety.
出处
《食品安全质量检测学报》
CAS
2014年第3期840-845,共6页
Journal of Food Safety and Quality
基金
国家公益性行业(农业)科研专项(201203094)
国家国际科技合作专项资助(2012DFA31140)
吉林省世行贷款农产品质量安全项目(2011-Z50)~~
关键词
化学发光免疫分析
食品安全
研究进展
chemiluminescent immunoassay
food safety
research progress
